Superinfection Drives HIV Neutralizing Antibody Responses from Several B Cell Lineages that Contribute to a Polyclonal Repertoire
Summary: Eliciting broad and potent HIV-specific neutralizing antibody responses represents the holy grail of HIV vaccine efforts. Data from singly infected individuals with broad and potent plasma neutralizing activity targeting one epitope have guided our understanding of how these responses devel...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-04-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124718304431 |
| _version_ | 1819087346861080576 |
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| author | Katherine L. Williams Bingjie Wang Dana Arenz James A. Williams Adam S. Dingens Valerie Cortez Cassandra A. Simonich Stephanie Rainwater Dara A. Lehman Kelly K. Lee Julie Overbaugh |
| author_facet | Katherine L. Williams Bingjie Wang Dana Arenz James A. Williams Adam S. Dingens Valerie Cortez Cassandra A. Simonich Stephanie Rainwater Dara A. Lehman Kelly K. Lee Julie Overbaugh |
| author_sort | Katherine L. Williams |
| collection | DOAJ |
| description | Summary: Eliciting broad and potent HIV-specific neutralizing antibody responses represents the holy grail of HIV vaccine efforts. Data from singly infected individuals with broad and potent plasma neutralizing activity targeting one epitope have guided our understanding of how these responses develop. However, far less is known about responses developed by superinfected individuals who acquire two distinct HIV strains. Here, we isolated HIV-specific mAbs from a superinfected individual with a broad plasma response. In this superinfection case, neutralizing activity resulted from multiple distinct B cell lineages that arose in response to either the initial or the superinfecting virus, including an antibody that targets the N332 supersite. This nAb, QA013.2, was specific to the superinfecting virus and was associated with eventual reemergence of the initial infecting virus. The complex dynamic between viruses in superinfection may drive development of a unique collection of polyclonal nAbs that present a higher barrier to escape than monoclonal responses. : Superinfection occurs when an HIV-infected person acquires a second infection with a genetically distinct HIV virus. Williams et al. isolate HIV-specific mAbs from a superinfected individual with a broad plasma response. In this superinfection case, neutralizing activity resulted from multiple distinct B cell lineages that arose in response to the initial or superinfecting virus, including an antibody that targets the N332 supersite. Keywords: HIV, antibody, broadly neutralizing antibody, HIV viral escape |
| first_indexed | 2024-12-21T21:34:41Z |
| format | Article |
| id | doaj.art-d2f932fc2bb3434597cf2cf86c15ef2e |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-21T21:34:41Z |
| publishDate | 2018-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-d2f932fc2bb3434597cf2cf86c15ef2e2022-12-21T18:49:32ZengElsevierCell Reports2211-12472018-04-01233682691Superinfection Drives HIV Neutralizing Antibody Responses from Several B Cell Lineages that Contribute to a Polyclonal RepertoireKatherine L. Williams0Bingjie Wang1Dana Arenz2James A. Williams3Adam S. Dingens4Valerie Cortez5Cassandra A. Simonich6Stephanie Rainwater7Dara A. Lehman8Kelly K. Lee9Julie Overbaugh10Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98195, USADivision of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98195, USA; Medical Scientist Training Program, University of Washington School of Medicine, Seattle, WA 98195, USADivision of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98195, USADepartment of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USADivision of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98195, USA; Program in Molecular and Cellular Biology, University of Washington, Seattle, WA 98195, USADivision of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98195, USA; Program in Molecular and Cellular Biology, University of Washington, Seattle, WA 98195, USADivision of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98195, USA; Medical Scientist Training Program, University of Washington School of Medicine, Seattle, WA 98195, USADivision of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98195, USADivision of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98195, USADepartment of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USADivision of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98195, USA; Corresponding authorSummary: Eliciting broad and potent HIV-specific neutralizing antibody responses represents the holy grail of HIV vaccine efforts. Data from singly infected individuals with broad and potent plasma neutralizing activity targeting one epitope have guided our understanding of how these responses develop. However, far less is known about responses developed by superinfected individuals who acquire two distinct HIV strains. Here, we isolated HIV-specific mAbs from a superinfected individual with a broad plasma response. In this superinfection case, neutralizing activity resulted from multiple distinct B cell lineages that arose in response to either the initial or the superinfecting virus, including an antibody that targets the N332 supersite. This nAb, QA013.2, was specific to the superinfecting virus and was associated with eventual reemergence of the initial infecting virus. The complex dynamic between viruses in superinfection may drive development of a unique collection of polyclonal nAbs that present a higher barrier to escape than monoclonal responses. : Superinfection occurs when an HIV-infected person acquires a second infection with a genetically distinct HIV virus. Williams et al. isolate HIV-specific mAbs from a superinfected individual with a broad plasma response. In this superinfection case, neutralizing activity resulted from multiple distinct B cell lineages that arose in response to the initial or superinfecting virus, including an antibody that targets the N332 supersite. Keywords: HIV, antibody, broadly neutralizing antibody, HIV viral escapehttp://www.sciencedirect.com/science/article/pii/S2211124718304431 |
| spellingShingle | Katherine L. Williams Bingjie Wang Dana Arenz James A. Williams Adam S. Dingens Valerie Cortez Cassandra A. Simonich Stephanie Rainwater Dara A. Lehman Kelly K. Lee Julie Overbaugh Superinfection Drives HIV Neutralizing Antibody Responses from Several B Cell Lineages that Contribute to a Polyclonal Repertoire Cell Reports |
| title | Superinfection Drives HIV Neutralizing Antibody Responses from Several B Cell Lineages that Contribute to a Polyclonal Repertoire |
| title_full | Superinfection Drives HIV Neutralizing Antibody Responses from Several B Cell Lineages that Contribute to a Polyclonal Repertoire |
| title_fullStr | Superinfection Drives HIV Neutralizing Antibody Responses from Several B Cell Lineages that Contribute to a Polyclonal Repertoire |
| title_full_unstemmed | Superinfection Drives HIV Neutralizing Antibody Responses from Several B Cell Lineages that Contribute to a Polyclonal Repertoire |
| title_short | Superinfection Drives HIV Neutralizing Antibody Responses from Several B Cell Lineages that Contribute to a Polyclonal Repertoire |
| title_sort | superinfection drives hiv neutralizing antibody responses from several b cell lineages that contribute to a polyclonal repertoire |
| url | http://www.sciencedirect.com/science/article/pii/S2211124718304431 |
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