Gypenosides Synergistically Reduce the Extracellular Matrix of Hepatic Stellate Cells and Ameliorate Hepatic Fibrosis in Mice
Liver fibrosis resulting from chronic liver damage is becoming one of the major threats to health worldwide. Active saponin constituents isolated from <i>Gynostemma pentaphyllum</i> were found to possess a protective effect in liver diseases. Here, we obtained a naturally abundant gypeno...
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MDPI AG
2023-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/14/5448 |
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| author | Han Li Hanghang Wang Aiping Yang Mingzhen Xue Junyang Wang Qi Lv Jian Liu Lihong Hu Yinan Zhang Xiachang Wang |
| author_facet | Han Li Hanghang Wang Aiping Yang Mingzhen Xue Junyang Wang Qi Lv Jian Liu Lihong Hu Yinan Zhang Xiachang Wang |
| author_sort | Han Li |
| collection | DOAJ |
| description | Liver fibrosis resulting from chronic liver damage is becoming one of the major threats to health worldwide. Active saponin constituents isolated from <i>Gynostemma pentaphyllum</i> were found to possess a protective effect in liver diseases. Here, we obtained a naturally abundant gypenoside, XLVI, and evaluated its liver protection activity in both animal and cellular models. The results showed that it ameliorated acute and chronic liver injuries and lightened the process of fibrogenesis in vivo. XLVI can inhibit TGF-β-induced activation of hepatic stellate cells and ECM deposition in vitro. The underlying mechanism study verified that it upregulated the protein expression of protein phosphatase 2C alpha and strengthened the vitality of the phosphatase together with a PP2Cα agonist gypenoside NPLC0393. These results shed new light on the molecular mechanisms and the potential therapeutic function of the traditional herb <i>Gynostemma pentaphyllum</i> in the treatment of liver fibrosis. |
| first_indexed | 2024-03-11T00:47:18Z |
| format | Article |
| id | doaj.art-d2fc395265354c0caff9e0a4721ae8ee |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T00:47:18Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d2fc395265354c0caff9e0a4721ae8ee2023-11-18T20:42:17ZengMDPI AGMolecules1420-30492023-07-012814544810.3390/molecules28145448Gypenosides Synergistically Reduce the Extracellular Matrix of Hepatic Stellate Cells and Ameliorate Hepatic Fibrosis in MiceHan Li0Hanghang Wang1Aiping Yang2Mingzhen Xue3Junyang Wang4Qi Lv5Jian Liu6Lihong Hu7Yinan Zhang8Xiachang Wang9Jiangsu Key Laboratory for Functional Substances of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Key Laboratory for Functional Substances of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Key Laboratory for Functional Substances of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Key Laboratory for Functional Substances of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Key Laboratory for Functional Substances of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Key Laboratory for Functional Substances of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Key Laboratory for Functional Substances of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Key Laboratory for Functional Substances of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Key Laboratory for Functional Substances of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Key Laboratory for Functional Substances of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaLiver fibrosis resulting from chronic liver damage is becoming one of the major threats to health worldwide. Active saponin constituents isolated from <i>Gynostemma pentaphyllum</i> were found to possess a protective effect in liver diseases. Here, we obtained a naturally abundant gypenoside, XLVI, and evaluated its liver protection activity in both animal and cellular models. The results showed that it ameliorated acute and chronic liver injuries and lightened the process of fibrogenesis in vivo. XLVI can inhibit TGF-β-induced activation of hepatic stellate cells and ECM deposition in vitro. The underlying mechanism study verified that it upregulated the protein expression of protein phosphatase 2C alpha and strengthened the vitality of the phosphatase together with a PP2Cα agonist gypenoside NPLC0393. These results shed new light on the molecular mechanisms and the potential therapeutic function of the traditional herb <i>Gynostemma pentaphyllum</i> in the treatment of liver fibrosis.https://www.mdpi.com/1420-3049/28/14/5448gypenosideliver fibrosisPP2Cαextracellular matrixsynergistic effect |
| spellingShingle | Han Li Hanghang Wang Aiping Yang Mingzhen Xue Junyang Wang Qi Lv Jian Liu Lihong Hu Yinan Zhang Xiachang Wang Gypenosides Synergistically Reduce the Extracellular Matrix of Hepatic Stellate Cells and Ameliorate Hepatic Fibrosis in Mice Molecules gypenoside liver fibrosis PP2Cα extracellular matrix synergistic effect |
| title | Gypenosides Synergistically Reduce the Extracellular Matrix of Hepatic Stellate Cells and Ameliorate Hepatic Fibrosis in Mice |
| title_full | Gypenosides Synergistically Reduce the Extracellular Matrix of Hepatic Stellate Cells and Ameliorate Hepatic Fibrosis in Mice |
| title_fullStr | Gypenosides Synergistically Reduce the Extracellular Matrix of Hepatic Stellate Cells and Ameliorate Hepatic Fibrosis in Mice |
| title_full_unstemmed | Gypenosides Synergistically Reduce the Extracellular Matrix of Hepatic Stellate Cells and Ameliorate Hepatic Fibrosis in Mice |
| title_short | Gypenosides Synergistically Reduce the Extracellular Matrix of Hepatic Stellate Cells and Ameliorate Hepatic Fibrosis in Mice |
| title_sort | gypenosides synergistically reduce the extracellular matrix of hepatic stellate cells and ameliorate hepatic fibrosis in mice |
| topic | gypenoside liver fibrosis PP2Cα extracellular matrix synergistic effect |
| url | https://www.mdpi.com/1420-3049/28/14/5448 |
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