Screening of Poly-Glutamic Acid (PGA)-Producing <i>Bacillus</i> Species from Indian Fermented Soybean Foods and Characterization of PGA
This present work is aimed to screen the PGA-producing <i>Bacillus</i> spp. from naturally fermented soybean foods of Arunachal Pradesh in India and to characterize the poly-glutamic acid (PGA) extracted from <i>Bacillus</i> spp. A total of 50 isolates were screened for high...
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MDPI AG
2022-09-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/10/495 |
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| author | Priyambada Pariyar Puneeta Singh Yaduvanshi Pullakhanadam Raghu Jyoti Prakash Tamang |
| author_facet | Priyambada Pariyar Puneeta Singh Yaduvanshi Pullakhanadam Raghu Jyoti Prakash Tamang |
| author_sort | Priyambada Pariyar |
| collection | DOAJ |
| description | This present work is aimed to screen the PGA-producing <i>Bacillus</i> spp. from naturally fermented soybean foods of Arunachal Pradesh in India and to characterize the poly-glutamic acid (PGA) extracted from <i>Bacillus</i> spp. A total of 50 isolates were screened for high stickiness from 165 bacterial isolates isolated from 34 samples <i>viz</i>., <i>grep chhurpi</i>, <i>peha/paeha</i>, <i>peron namsing</i>, and <i>peruñyaan</i>. Based on 16S rRNA gene sequence analysis, 50 isolates were identified as <i>Bacillus</i> spp. Viscosity of 50 species of <i>Bacillus</i> were measured, out of which 7 species of <i>Bacillus</i> showing ≥0.03 Pa.s (30 cP) of viscosity were selected <i>viz</i>., <i>Bacillus velezensis</i> GC1-42, <i>B. siamensis</i> GC4-36, <i>B. subtilis</i> PH3-21, <i>B. subtilis</i> PN1-14, <i>B. subtilis</i> PH6-29, <i>B. tequilensis</i> PN9-22, and <i>B. safensis</i> subsp. <i>safensis</i> PY1-19 for PGA production. Amino acid analysis of PGA extracted from seven species of <i>Bacillus</i> showed high molecular weight (>600 kDa). <i>B. safensis</i> subsp. <i>safensis</i> PY1-19, isolated from <i>peruñyaan</i>, showed 78.9% of glutamic acid, antagonistic properties against pathogenic bacteria and had the ability to produce phytase with no hemolytic activity. Hence, this strain was selected as a potential starter to prepare <i>peruñyaan</i> at laboratory, and the final product showed high stickiness and viscosity with production of PGA of around 11 g/L. |
| first_indexed | 2024-03-09T12:20:14Z |
| format | Article |
| id | doaj.art-d2ff0453df4a45109482423365de2450 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T12:20:14Z |
| publishDate | 2022-09-01 |
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| series | Fermentation |
| spelling | doaj.art-d2ff0453df4a45109482423365de24502023-11-30T22:41:56ZengMDPI AGFermentation2311-56372022-09-0181049510.3390/fermentation8100495Screening of Poly-Glutamic Acid (PGA)-Producing <i>Bacillus</i> Species from Indian Fermented Soybean Foods and Characterization of PGAPriyambada Pariyar0Puneeta Singh Yaduvanshi1Pullakhanadam Raghu2Jyoti Prakash Tamang3Department of Microbiology, School of Life Sciences, Sikkim University, Gangtok 737102, IndiaMicronutrient Research Division, ICMR-National Institute of Nutrition, Osmania, Hyderabad 500007, IndiaMicronutrient Research Division, ICMR-National Institute of Nutrition, Osmania, Hyderabad 500007, IndiaDepartment of Microbiology, School of Life Sciences, Sikkim University, Gangtok 737102, IndiaThis present work is aimed to screen the PGA-producing <i>Bacillus</i> spp. from naturally fermented soybean foods of Arunachal Pradesh in India and to characterize the poly-glutamic acid (PGA) extracted from <i>Bacillus</i> spp. A total of 50 isolates were screened for high stickiness from 165 bacterial isolates isolated from 34 samples <i>viz</i>., <i>grep chhurpi</i>, <i>peha/paeha</i>, <i>peron namsing</i>, and <i>peruñyaan</i>. Based on 16S rRNA gene sequence analysis, 50 isolates were identified as <i>Bacillus</i> spp. Viscosity of 50 species of <i>Bacillus</i> were measured, out of which 7 species of <i>Bacillus</i> showing ≥0.03 Pa.s (30 cP) of viscosity were selected <i>viz</i>., <i>Bacillus velezensis</i> GC1-42, <i>B. siamensis</i> GC4-36, <i>B. subtilis</i> PH3-21, <i>B. subtilis</i> PN1-14, <i>B. subtilis</i> PH6-29, <i>B. tequilensis</i> PN9-22, and <i>B. safensis</i> subsp. <i>safensis</i> PY1-19 for PGA production. Amino acid analysis of PGA extracted from seven species of <i>Bacillus</i> showed high molecular weight (>600 kDa). <i>B. safensis</i> subsp. <i>safensis</i> PY1-19, isolated from <i>peruñyaan</i>, showed 78.9% of glutamic acid, antagonistic properties against pathogenic bacteria and had the ability to produce phytase with no hemolytic activity. Hence, this strain was selected as a potential starter to prepare <i>peruñyaan</i> at laboratory, and the final product showed high stickiness and viscosity with production of PGA of around 11 g/L.https://www.mdpi.com/2311-5637/8/10/495fermented soybeanpolyglutamic acid<i>Bacillus</i> spp.<i>B. safensis</i> subsp. <i>safensis</i>starter culture |
| spellingShingle | Priyambada Pariyar Puneeta Singh Yaduvanshi Pullakhanadam Raghu Jyoti Prakash Tamang Screening of Poly-Glutamic Acid (PGA)-Producing <i>Bacillus</i> Species from Indian Fermented Soybean Foods and Characterization of PGA Fermentation fermented soybean polyglutamic acid <i>Bacillus</i> spp. <i>B. safensis</i> subsp. <i>safensis</i> starter culture |
| title | Screening of Poly-Glutamic Acid (PGA)-Producing <i>Bacillus</i> Species from Indian Fermented Soybean Foods and Characterization of PGA |
| title_full | Screening of Poly-Glutamic Acid (PGA)-Producing <i>Bacillus</i> Species from Indian Fermented Soybean Foods and Characterization of PGA |
| title_fullStr | Screening of Poly-Glutamic Acid (PGA)-Producing <i>Bacillus</i> Species from Indian Fermented Soybean Foods and Characterization of PGA |
| title_full_unstemmed | Screening of Poly-Glutamic Acid (PGA)-Producing <i>Bacillus</i> Species from Indian Fermented Soybean Foods and Characterization of PGA |
| title_short | Screening of Poly-Glutamic Acid (PGA)-Producing <i>Bacillus</i> Species from Indian Fermented Soybean Foods and Characterization of PGA |
| title_sort | screening of poly glutamic acid pga producing i bacillus i species from indian fermented soybean foods and characterization of pga |
| topic | fermented soybean polyglutamic acid <i>Bacillus</i> spp. <i>B. safensis</i> subsp. <i>safensis</i> starter culture |
| url | https://www.mdpi.com/2311-5637/8/10/495 |
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