Affinity chromatography and capillary electrophoresis for analysis of the yeast ribosomal proteins

Affinity chromatography and capillary electrophoresis for analysis of the yeast ribosomal proteins

We present a top down separation platform for yeast ribosomalproteins using affinity chromatography and capillary electrophoresiswhich is designed to allow deposition of proteins ontoa substrate. FLAG tagged ribosomes were affinity purified, andrRNA acid precipitation was performed on the ribosomes...

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Bibliographic Details
Main Authors: Miriam S. Goyder, Keith R. Willison, David R. Klug, Andrew J. deMello, Oscar Ces
Format: Article
Language:English
Published: Korean Society for Biochemistry and Molecular Biology 2012-04-01
Series:BMB Reports
Subjects:
Affinity chromatography
Capillary electrophoresis
EVV 2DIR
Proteomics
Ribosomal proteins
Online Access:http://bmbreports.org/jbmb/pdf.php?data=MTMxMTA4MTZAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS00JTVEMTIwNDI2MTkwN18lMjgyMzMtMjM4JTI5Qk1CXzExLTI2OS5wZGY=

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http://bmbreports.org/jbmb/pdf.php?data=MTMxMTA4MTZAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS00JTVEMTIwNDI2MTkwN18lMjgyMzMtMjM4JTI5Qk1CXzExLTI2OS5wZGY=

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