CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation
Microtubules are dynamic polymers consisting of αβ-tubulin heterodimers. The initial polymerization process, called microtubule nucleation, occurs spontaneously via αβ-tubulin. Since a large energy barrier prevents microtubule nucleation in cells, the γ-tubulin ring complex is recruited to the centr...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2022-06-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/77365 |
| _version_ | 1811203577846169600 |
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| author | Tsuyoshi Imasaki Satoshi Kikkawa Shinsuke Niwa Yumiko Saijo-Hamano Hideki Shigematsu Kazuhiro Aoyama Kaoru Mitsuoka Takahiro Shimizu Mari Aoki Ayako Sakamoto Yuri Tomabechi Naoki Sakai Mikako Shirouzu Shinya Taguchi Yosuke Yamagishi Tomiyoshi Setsu Yoshiaki Sakihama Eriko Nitta Masatoshi Takeichi Ryo Nitta |
| author_facet | Tsuyoshi Imasaki Satoshi Kikkawa Shinsuke Niwa Yumiko Saijo-Hamano Hideki Shigematsu Kazuhiro Aoyama Kaoru Mitsuoka Takahiro Shimizu Mari Aoki Ayako Sakamoto Yuri Tomabechi Naoki Sakai Mikako Shirouzu Shinya Taguchi Yosuke Yamagishi Tomiyoshi Setsu Yoshiaki Sakihama Eriko Nitta Masatoshi Takeichi Ryo Nitta |
| author_sort | Tsuyoshi Imasaki |
| collection | DOAJ |
| description | Microtubules are dynamic polymers consisting of αβ-tubulin heterodimers. The initial polymerization process, called microtubule nucleation, occurs spontaneously via αβ-tubulin. Since a large energy barrier prevents microtubule nucleation in cells, the γ-tubulin ring complex is recruited to the centrosome to overcome the nucleation barrier. However, a considerable number of microtubules can polymerize independently of the centrosome in various cell types. Here, we present evidence that the minus-end-binding calmodulin-regulated spectrin-associated protein 2 (CAMSAP2) serves as a strong nucleator for microtubule formation by significantly reducing the nucleation barrier. CAMSAP2 co-condensates with αβ-tubulin via a phase separation process, producing plenty of nucleation intermediates. Microtubules then radiate from the co-condensates, resulting in aster-like structure formation. CAMSAP2 localizes at the co-condensates and decorates the radiating microtubule lattices to some extent. Taken together, these in vitro findings suggest that CAMSAP2 supports microtubule nucleation and growth by organizing a nucleation centre as well as by stabilizing microtubule intermediates and growing microtubules. |
| first_indexed | 2024-04-12T02:57:40Z |
| format | Article |
| id | doaj.art-d32eeac96c0645ad83b454410aa41fe9 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:57:40Z |
| publishDate | 2022-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-d32eeac96c0645ad83b454410aa41fe92022-12-22T03:50:46ZengeLife Sciences Publications LtdeLife2050-084X2022-06-011110.7554/eLife.77365CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separationTsuyoshi Imasaki0https://orcid.org/0000-0001-5462-1820Satoshi Kikkawa1Shinsuke Niwa2Yumiko Saijo-Hamano3Hideki Shigematsu4https://orcid.org/0000-0003-3951-8651Kazuhiro Aoyama5Kaoru Mitsuoka6https://orcid.org/0000-0003-1782-675XTakahiro Shimizu7Mari Aoki8Ayako Sakamoto9Yuri Tomabechi10Naoki Sakai11Mikako Shirouzu12https://orcid.org/0000-0002-7997-2149Shinya Taguchi13https://orcid.org/0000-0002-9868-0651Yosuke Yamagishi14Tomiyoshi Setsu15Yoshiaki Sakihama16Eriko Nitta17Masatoshi Takeichi18Ryo Nitta19https://orcid.org/0000-0002-6537-9272Division of Structural Medicine and Anatomy, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe, Japan; JST, PRESTO, Saitama, Japan; RIKEN Center for Biosystems Dynamics Research, Yokohama, JapanDivision of Structural Medicine and Anatomy, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe, JapanFrontier Research Institute for Interdisciplinary Sciences, Tohoku University, Sendai, JapanDivision of Structural Medicine and Anatomy, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe, JapanRIKEN SPring-8 Center, Hyogo, Japan; Japan Synchrotron Radiation Research Institute (JASRI), Hyogo, JapanMaterials and Structural Analysis, Thermo Fisher Scientific, Tokyo, Japan; Research Center for Ultra-High Voltage Electron Microscopy, Osaka University, Osaka, JapanResearch Center for Ultra-High Voltage Electron Microscopy, Osaka University, Osaka, JapanDivision of Structural Medicine and Anatomy, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe, JapanRIKEN Center for Biosystems Dynamics Research, Yokohama, JapanRIKEN Center for Biosystems Dynamics Research, Yokohama, JapanRIKEN Center for Biosystems Dynamics Research, Yokohama, JapanRIKEN SPring-8 Center, Hyogo, Japan; Japan Synchrotron Radiation Research Institute (JASRI), Hyogo, JapanRIKEN Center for Biosystems Dynamics Research, Yokohama, JapanDivision of Structural Medicine and Anatomy, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe, JapanDivision of Structural Medicine and Anatomy, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe, JapanDivision of Structural Medicine and Anatomy, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe, JapanDivision of Structural Medicine and Anatomy, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe, JapanDivision of Structural Medicine and Anatomy, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe, JapanRIKEN Center for Biosystems Dynamics Research, Kobe, JapanDivision of Structural Medicine and Anatomy, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe, Japan; RIKEN Center for Biosystems Dynamics Research, Yokohama, JapanMicrotubules are dynamic polymers consisting of αβ-tubulin heterodimers. The initial polymerization process, called microtubule nucleation, occurs spontaneously via αβ-tubulin. Since a large energy barrier prevents microtubule nucleation in cells, the γ-tubulin ring complex is recruited to the centrosome to overcome the nucleation barrier. However, a considerable number of microtubules can polymerize independently of the centrosome in various cell types. Here, we present evidence that the minus-end-binding calmodulin-regulated spectrin-associated protein 2 (CAMSAP2) serves as a strong nucleator for microtubule formation by significantly reducing the nucleation barrier. CAMSAP2 co-condensates with αβ-tubulin via a phase separation process, producing plenty of nucleation intermediates. Microtubules then radiate from the co-condensates, resulting in aster-like structure formation. CAMSAP2 localizes at the co-condensates and decorates the radiating microtubule lattices to some extent. Taken together, these in vitro findings suggest that CAMSAP2 supports microtubule nucleation and growth by organizing a nucleation centre as well as by stabilizing microtubule intermediates and growing microtubules.https://elifesciences.org/articles/77365microtubuleCAMSAPcryo-EMTIRFnucleationLLPS |
| spellingShingle | Tsuyoshi Imasaki Satoshi Kikkawa Shinsuke Niwa Yumiko Saijo-Hamano Hideki Shigematsu Kazuhiro Aoyama Kaoru Mitsuoka Takahiro Shimizu Mari Aoki Ayako Sakamoto Yuri Tomabechi Naoki Sakai Mikako Shirouzu Shinya Taguchi Yosuke Yamagishi Tomiyoshi Setsu Yoshiaki Sakihama Eriko Nitta Masatoshi Takeichi Ryo Nitta CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation eLife microtubule CAMSAP cryo-EM TIRF nucleation LLPS |
| title | CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation |
| title_full | CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation |
| title_fullStr | CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation |
| title_full_unstemmed | CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation |
| title_short | CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation |
| title_sort | camsap2 organizes a γ tubulin independent microtubule nucleation centre through phase separation |
| topic | microtubule CAMSAP cryo-EM TIRF nucleation LLPS |
| url | https://elifesciences.org/articles/77365 |
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