Engineered Type Six Secretion Systems Deliver Active Exogenous Effectors and Cre Recombinase
ABSTRACT Genetic editing has revolutionized biotechnology, but delivery of endonuclease genes as DNA can lead to aberrant integration or overexpression, leading to off-target effects. Here, we develop a mechanism to deliver Cre recombinase as a protein by engineering the bacterial type six secretion...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2021-08-01
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| Series: | mBio |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/mBio.01115-21 |