Detection of Enterotoxigenic Potential and Determination of Clonal Profile in Staphylococcus aureus and Coagulase-Negative Staphylococci Isolated from Bovine Subclinical Mastitis in Different Brazilian States
Epidemiological studies have identified Staphylococcus aureus as the most common agent involved in food poisoning. However, current research highlights the importance of toxigenic coagulase-negative staphylococci (CoNS) isolated from food. The aim of this study was to characterize Staphylococcus spp...
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MDPI AG
2016-04-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/8/4/104 |
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| author | Priscila Luiza Mello Danilo Flávio Moraes Riboli Luiza Pinheiro Lisiane de Almeida Martins Maria Aparecida Vasconcelos Paiva Brito Maria de Lourdes Ribeiro de Souza da Cunha |
| author_facet | Priscila Luiza Mello Danilo Flávio Moraes Riboli Luiza Pinheiro Lisiane de Almeida Martins Maria Aparecida Vasconcelos Paiva Brito Maria de Lourdes Ribeiro de Souza da Cunha |
| author_sort | Priscila Luiza Mello |
| collection | DOAJ |
| description | Epidemiological studies have identified Staphylococcus aureus as the most common agent involved in food poisoning. However, current research highlights the importance of toxigenic coagulase-negative staphylococci (CoNS) isolated from food. The aim of this study was to characterize Staphylococcus spp. isolated from cows with bovine subclinical mastitis regarding the presence of genes responsible for the production of staphylococcal enterotoxins and of the tst-1 gene encoding toxic shock syndrome toxin 1, and to determine the clonal profile of the isolates carrying any of the genes studied. A total of 181 strains isolated in different Brazilian states, including the South, Southeast, and Northeast regions, were analyzed. The sea gene was the most frequent, which was detected in 18.2% of the isolates, followed by seb in 7.7%, sec in 14.9%, sed in 0.5%, see in 8.2%, seg in 1.6%, seh in 25.4%, sei in 6.6%, and ser in 1.6%. The sej, ses, set, and tst-1 genes were not detected in any of the isolates. The typing of the isolates by pulsed-field gel electrophoresis revealed important S. aureus and S. epidermidis clusters in different areas and the presence of enterotoxin genes in lineages isolated from animals that belong to herds located geographically close to each other. |
| first_indexed | 2024-04-14T02:29:10Z |
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| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-14T02:29:10Z |
| publishDate | 2016-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-d3710f5647e245f394a04501adc794fc2022-12-22T02:17:45ZengMDPI AGToxins2072-66512016-04-018410410.3390/toxins8040104toxins8040104Detection of Enterotoxigenic Potential and Determination of Clonal Profile in Staphylococcus aureus and Coagulase-Negative Staphylococci Isolated from Bovine Subclinical Mastitis in Different Brazilian StatesPriscila Luiza Mello0Danilo Flávio Moraes Riboli1Luiza Pinheiro2Lisiane de Almeida Martins3Maria Aparecida Vasconcelos Paiva Brito4Maria de Lourdes Ribeiro de Souza da Cunha5Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu 18618-970, BrazilDepartment of Microbiology and Immunology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu 18618-970, BrazilDepartment of Microbiology and Immunology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu 18618-970, BrazilUniversidade Paranaense-UNIPAR, Umuarama 87502-210, BrazilEmbrapa Dairy Cattle, Juiz de Fora 36038-330, BrazilDepartment of Microbiology and Immunology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu 18618-970, BrazilEpidemiological studies have identified Staphylococcus aureus as the most common agent involved in food poisoning. However, current research highlights the importance of toxigenic coagulase-negative staphylococci (CoNS) isolated from food. The aim of this study was to characterize Staphylococcus spp. isolated from cows with bovine subclinical mastitis regarding the presence of genes responsible for the production of staphylococcal enterotoxins and of the tst-1 gene encoding toxic shock syndrome toxin 1, and to determine the clonal profile of the isolates carrying any of the genes studied. A total of 181 strains isolated in different Brazilian states, including the South, Southeast, and Northeast regions, were analyzed. The sea gene was the most frequent, which was detected in 18.2% of the isolates, followed by seb in 7.7%, sec in 14.9%, sed in 0.5%, see in 8.2%, seg in 1.6%, seh in 25.4%, sei in 6.6%, and ser in 1.6%. The sej, ses, set, and tst-1 genes were not detected in any of the isolates. The typing of the isolates by pulsed-field gel electrophoresis revealed important S. aureus and S. epidermidis clusters in different areas and the presence of enterotoxin genes in lineages isolated from animals that belong to herds located geographically close to each other.http://www.mdpi.com/2072-6651/8/4/104staphylococcal enterotoxinbovine mammary glandBrazilian Statesmastitispulsed-field gel electrophoresisclonal profile |
| spellingShingle | Priscila Luiza Mello Danilo Flávio Moraes Riboli Luiza Pinheiro Lisiane de Almeida Martins Maria Aparecida Vasconcelos Paiva Brito Maria de Lourdes Ribeiro de Souza da Cunha Detection of Enterotoxigenic Potential and Determination of Clonal Profile in Staphylococcus aureus and Coagulase-Negative Staphylococci Isolated from Bovine Subclinical Mastitis in Different Brazilian States Toxins staphylococcal enterotoxin bovine mammary gland Brazilian States mastitis pulsed-field gel electrophoresis clonal profile |
| title | Detection of Enterotoxigenic Potential and Determination of Clonal Profile in Staphylococcus aureus and Coagulase-Negative Staphylococci Isolated from Bovine Subclinical Mastitis in Different Brazilian States |
| title_full | Detection of Enterotoxigenic Potential and Determination of Clonal Profile in Staphylococcus aureus and Coagulase-Negative Staphylococci Isolated from Bovine Subclinical Mastitis in Different Brazilian States |
| title_fullStr | Detection of Enterotoxigenic Potential and Determination of Clonal Profile in Staphylococcus aureus and Coagulase-Negative Staphylococci Isolated from Bovine Subclinical Mastitis in Different Brazilian States |
| title_full_unstemmed | Detection of Enterotoxigenic Potential and Determination of Clonal Profile in Staphylococcus aureus and Coagulase-Negative Staphylococci Isolated from Bovine Subclinical Mastitis in Different Brazilian States |
| title_short | Detection of Enterotoxigenic Potential and Determination of Clonal Profile in Staphylococcus aureus and Coagulase-Negative Staphylococci Isolated from Bovine Subclinical Mastitis in Different Brazilian States |
| title_sort | detection of enterotoxigenic potential and determination of clonal profile in staphylococcus aureus and coagulase negative staphylococci isolated from bovine subclinical mastitis in different brazilian states |
| topic | staphylococcal enterotoxin bovine mammary gland Brazilian States mastitis pulsed-field gel electrophoresis clonal profile |
| url | http://www.mdpi.com/2072-6651/8/4/104 |
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