Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair

Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair

The CRISPR-Cas9 targeted nuclease technology allows the insertion of genetic modifications with single base-pair precision. The preference of mammalian cells to repair Cas9-induced DNA double-strand breaks via error-prone end-joining pathways rather than via homology-directed repair mechanisms, howe...

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Bibliographic Details
Main Authors: Natasa Savic, Femke CAS Ringnalda, Helen Lindsay, Christian Berk, Katja Bargsten, Yizhou Li, Dario Neri, Mark D Robinson, Constance Ciaudo, Jonathan Hall, Martin Jinek, Gerald Schwank
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2018-05-01
Series:eLife
Subjects:
CRISPR/Cas9
gene editing
homology-directed repair
Online Access:https://elifesciences.org/articles/33761

Internet

https://elifesciences.org/articles/33761

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