A Sensitive LC-MS/MS Method for the Simultaneous Determination of Two Thia-Analogous Indirubin <i>N</i>-Glycosides and Indirubin-3′-Monoxime in Plasma and Cell Culture Medium

A Sensitive LC-MS/MS Method for the Simultaneous Determination of Two Thia-Analogous Indirubin <i>N</i>-Glycosides and Indirubin-3′-Monoxime in Plasma and Cell Culture Medium

Indirubin was identified as an active component of Danggui Longhui Wan, an herbal mixture used in traditional Chinese medicine, and showed anticancer activity in clinical trials in patients with chronic leukemia. Investigations on the mechanisms of antitumor action of indirubins have mainly focused...

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Bibliographic Details
Main Authors: Alica Fischle, Rico Schwarz, Franziska Wendt, Marcel Kordt, Robert Ramer, Lars Boeckmann, Martin Hein, Peter Langer, Steffen Emmert, Brigitte Vollmar, Burkhard Hinz
Format: Article
Language:English
Published: MDPI AG 2022-05-01
Series:Molecules
Subjects:
thia-analogous indirubin <i>N</i>-glycosides
validation
protein precipitation
liquid-liquid extraction
LC-MS/MS
Online Access:https://www.mdpi.com/1420-3049/27/9/3031

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https://www.mdpi.com/1420-3049/27/9/3031

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