Production of Extracellular Protease from Bacterial Co-cultures using Solid State Fermentation
Proteases (also known as peptidases or proteinases) are hydrolytic enzymes that cleave proteins into amino acids. They comprise 60% of the total industrial usage of enzymes worldwide and can be obtained from many sources. The current study aims to isolate and screen protease-producing bacterial str...
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| Format: | Article |
| Language: | English |
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University of Management and Technology
2020-12-01
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| Series: | Bioscientific Review |
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| Online Access: | https://journals.umt.edu.pk/index.php/BSR/article/view/726 |
| _version_ | 1811240546412265472 |
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| author | Nabiha Naeem Sheikhs Qurat-ul-ain Saba Altaf |
| author_facet | Nabiha Naeem Sheikhs Qurat-ul-ain Saba Altaf |
| author_sort | Nabiha Naeem Sheikhs |
| collection | DOAJ |
| description |
Proteases (also known as peptidases or proteinases) are hydrolytic enzymes that cleave proteins into amino acids. They comprise 60% of the total industrial usage of enzymes worldwide and can be obtained from many sources. The current study aims to isolate and screen protease-producing bacterial strains from the soil and to produce protease from the bacterial co-cultures using solid-state fermentation (SSF). Primary screening of the protease-producing bacterial strains was carried out on skim milk agar and they were sub-cultured and preserved on the nutrient agar for further testing. Thirty-two compatibility tests of twenty-seven bacterial isolates were performed and SSF was carried out. Afterward, absorbance was taken at 660 nm against tyrosine as standard. According to the results, the bacterial co-culture 19 showed the highest absorbance with an enzyme activity of 10.2 U/ml. The bacterial strains of the co-culture 19 were identified through morphological and biochemical tests. Bacterial strain 1 was observed as cocci and irregular, while bacterial strain 2 was bacillus and rod-shaped. Both strains were positive for gram staining, catalase test, casein hydrolysis test and methyl red test. As for endospore staining, bacterial strain 1 was spore forming while bacterial strain 2 was a non-spore former. It was concluded that the bacterial co-culture 19 can act as a potent co-culture for protease production. Compatibility test was carried out to enhance the production of protease by utilizing cheap and readily available agro-waste products, which benefit the industry by being cost effective and the environment by being eco-friendly.
Copyright (c) The Authors
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| first_indexed | 2024-04-12T13:22:12Z |
| format | Article |
| id | doaj.art-d39c2a6e499a4b60b54dd3762f4758f6 |
| institution | Directory Open Access Journal |
| issn | 2663-4198 2663-4201 |
| language | English |
| last_indexed | 2024-04-12T13:22:12Z |
| publishDate | 2020-12-01 |
| publisher | University of Management and Technology |
| record_format | Article |
| series | Bioscientific Review |
| spelling | doaj.art-d39c2a6e499a4b60b54dd3762f4758f62022-12-22T03:31:27ZengUniversity of Management and TechnologyBioscientific Review2663-41982663-42012020-12-012410.32350/BSR.0204.02Production of Extracellular Protease from Bacterial Co-cultures using Solid State FermentationNabiha Naeem Sheikhs0Qurat-ul-ain1Saba Altaf2Department of Life Sciences, School of Science, University of Management and Technology, Lahore, PakistanDepartment of Life Sciences, School of Science, University of Management and Technology, Lahore, PakistanDepartment of Life Sciences, School of Science, University of Management and Technology, Lahore, Pakistan Proteases (also known as peptidases or proteinases) are hydrolytic enzymes that cleave proteins into amino acids. They comprise 60% of the total industrial usage of enzymes worldwide and can be obtained from many sources. The current study aims to isolate and screen protease-producing bacterial strains from the soil and to produce protease from the bacterial co-cultures using solid-state fermentation (SSF). Primary screening of the protease-producing bacterial strains was carried out on skim milk agar and they were sub-cultured and preserved on the nutrient agar for further testing. Thirty-two compatibility tests of twenty-seven bacterial isolates were performed and SSF was carried out. Afterward, absorbance was taken at 660 nm against tyrosine as standard. According to the results, the bacterial co-culture 19 showed the highest absorbance with an enzyme activity of 10.2 U/ml. The bacterial strains of the co-culture 19 were identified through morphological and biochemical tests. Bacterial strain 1 was observed as cocci and irregular, while bacterial strain 2 was bacillus and rod-shaped. Both strains were positive for gram staining, catalase test, casein hydrolysis test and methyl red test. As for endospore staining, bacterial strain 1 was spore forming while bacterial strain 2 was a non-spore former. It was concluded that the bacterial co-culture 19 can act as a potent co-culture for protease production. Compatibility test was carried out to enhance the production of protease by utilizing cheap and readily available agro-waste products, which benefit the industry by being cost effective and the environment by being eco-friendly. Copyright (c) The Authors https://journals.umt.edu.pk/index.php/BSR/article/view/726bacterial co-cultureenzyme assayshydrolytic enzymesproteasessolid-state fermentation |
| spellingShingle | Nabiha Naeem Sheikhs Qurat-ul-ain Saba Altaf Production of Extracellular Protease from Bacterial Co-cultures using Solid State Fermentation Bioscientific Review bacterial co-culture enzyme assays hydrolytic enzymes proteases solid-state fermentation |
| title | Production of Extracellular Protease from Bacterial Co-cultures using Solid State Fermentation |
| title_full | Production of Extracellular Protease from Bacterial Co-cultures using Solid State Fermentation |
| title_fullStr | Production of Extracellular Protease from Bacterial Co-cultures using Solid State Fermentation |
| title_full_unstemmed | Production of Extracellular Protease from Bacterial Co-cultures using Solid State Fermentation |
| title_short | Production of Extracellular Protease from Bacterial Co-cultures using Solid State Fermentation |
| title_sort | production of extracellular protease from bacterial co cultures using solid state fermentation |
| topic | bacterial co-culture enzyme assays hydrolytic enzymes proteases solid-state fermentation |
| url | https://journals.umt.edu.pk/index.php/BSR/article/view/726 |
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