Protocol to characterize extracellular c-Src tyrosine kinase function through substrate interaction and phosphorylation

Summary: Cellular Src tyrosine kinase (c-Src) exists in the secretomes of several human cancers (extracellular, e-Src). Phosphoproteomics has demonstrated the existence of 114 potential extracellular e-Src substrates in addition to Tissue Inhibitor of Metalloproteinases 2. Here, we present a protoco...

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Bibliographic Details
Main Authors: Michael P. Omini, Deema Alsalih, SarahBeth D. Votra, Dimitra Bourboulia
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723007220