Development of a rapid reverse genetics system for feline coronavirus based on TAR cloning in yeast

IntroductionReverse genetics has become an indispensable tool to gain insight into the pathogenesis of viruses and the development of vaccines. The yeast-based synthetic genomics platform has demonstrated the novel capabilities to genetically reconstruct different viruses.MethodsIn this study, a tra...

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Bibliographic Details
Main Authors: Hongmin Cao, Haorong Gu, Hongtao Kang, Honglin Jia
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-03-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2023.1141101/full