Design and Construction of Portable CRISPR-Cpf1-Mediated Genome Editing in Bacillus subtilis 168 Oriented Toward Multiple Utilities

Design and Construction of Portable CRISPR-Cpf1-Mediated Genome Editing in Bacillus subtilis 168 Oriented Toward Multiple Utilities

Bacillus subtilis is an important Gram-positive bacterium for industrial biotechnology, which has been widely used to produce diverse high-value added chemicals and industrially and pharmaceutically relevant proteins. Robust and versatile toolkits for genome editing in B. subtilis are highly demandi...

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Bibliographic Details
Main Authors: Wenliang Hao, Feiya Suo, Qiao Lin, Qiaoqing Chen, Li Zhou, Zhongmei Liu, Wenjing Cui, Zhemin Zhou
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-09-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
CRISPR-Cpf1
Bacillus subtilis
multiplex genome editing
large fragment deletion
gene insertion
chassis microorganisms
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2020.524676/full

Internet

https://www.frontiersin.org/article/10.3389/fbioe.2020.524676/full

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