The Protective Effect of Polygonum cuspidatum (PCE) Aqueous Extract in a Dry Eye Model
Dry eyes are caused by highly increased osmolarity of tear film, inflammation, and apoptosis of the ocular surface. In this study, we investigated the effect of Polygonum cuspidatum (PCE) aqueous extract in in vivo and in vitro dry eye models. Dry eye was induced by excision of the lacrimal gland an...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-10-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-6643/10/10/1550 |
| _version_ | 1811283030584590336 |
|---|---|
| author | Bongkyun Park Ik Soo Lee Soo-Wang Hyun Kyuhyung Jo Tae Gu Lee Jin Sook Kim Chan-Sik Kim |
| author_facet | Bongkyun Park Ik Soo Lee Soo-Wang Hyun Kyuhyung Jo Tae Gu Lee Jin Sook Kim Chan-Sik Kim |
| author_sort | Bongkyun Park |
| collection | DOAJ |
| description | Dry eyes are caused by highly increased osmolarity of tear film, inflammation, and apoptosis of the ocular surface. In this study, we investigated the effect of Polygonum cuspidatum (PCE) aqueous extract in in vivo and in vitro dry eye models. Dry eye was induced by excision of the lacrimal gland and hyperosmotic media. In vivo, oral administration of PCE in exorbital lacrimal gland-excised rats recovered tear volume and Mucin4 (MUC4) expression by inhibiting corneal irregularity and expression of inflammatory cytokines. In vitro, hyperosmotic media induced human corneal epithelial cell (HCEC) cytotoxicity though increased inflammation, apoptosis, and oxidative stress. PCE treatment significantly inhibited expression of cyclooxygenase-2 and inflammatory cytokines (interleukin-6 and tumor necrosis factor-α), and activation of NF-κB p65 in hyperosmolar stress-induced HCECs. Hyperosmolarity-induced increase in Bcl-2-associated X protein (BAX) expression and activation of cleaved poly (ADP-ribose) polymerase and caspase 3 were attenuated in a concentration-dependent manner by PCE. PCE treatment restored anti-oxidative proteins such as heme oxygenase-1 (HO-1), superoxide dismutase-1 (SOD-1), and glutathione peroxidase (GPx) in hyperosmolar stress-induced HCECs. These data demonstrate that PCE prevents adverse changes in the ocular surface and tear fluid through inhibition of hyperosmolar stress-induced inflammation, apoptosis, and oxidation, suggesting that PCE may have the potential to preserve eye health. |
| first_indexed | 2024-04-13T02:04:21Z |
| format | Article |
| id | doaj.art-d466a80400a24e9786e36b483c6a8f4d |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-04-13T02:04:21Z |
| publishDate | 2018-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-d466a80400a24e9786e36b483c6a8f4d2022-12-22T03:07:32ZengMDPI AGNutrients2072-66432018-10-011010155010.3390/nu10101550nu10101550The Protective Effect of Polygonum cuspidatum (PCE) Aqueous Extract in a Dry Eye ModelBongkyun Park0Ik Soo Lee1Soo-Wang Hyun2Kyuhyung Jo3Tae Gu Lee4Jin Sook Kim5Chan-Sik Kim6Clinical Medicine Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaHerbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaHerbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaClinical Medicine Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaClinical Medicine Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaHerbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaClinical Medicine Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaDry eyes are caused by highly increased osmolarity of tear film, inflammation, and apoptosis of the ocular surface. In this study, we investigated the effect of Polygonum cuspidatum (PCE) aqueous extract in in vivo and in vitro dry eye models. Dry eye was induced by excision of the lacrimal gland and hyperosmotic media. In vivo, oral administration of PCE in exorbital lacrimal gland-excised rats recovered tear volume and Mucin4 (MUC4) expression by inhibiting corneal irregularity and expression of inflammatory cytokines. In vitro, hyperosmotic media induced human corneal epithelial cell (HCEC) cytotoxicity though increased inflammation, apoptosis, and oxidative stress. PCE treatment significantly inhibited expression of cyclooxygenase-2 and inflammatory cytokines (interleukin-6 and tumor necrosis factor-α), and activation of NF-κB p65 in hyperosmolar stress-induced HCECs. Hyperosmolarity-induced increase in Bcl-2-associated X protein (BAX) expression and activation of cleaved poly (ADP-ribose) polymerase and caspase 3 were attenuated in a concentration-dependent manner by PCE. PCE treatment restored anti-oxidative proteins such as heme oxygenase-1 (HO-1), superoxide dismutase-1 (SOD-1), and glutathione peroxidase (GPx) in hyperosmolar stress-induced HCECs. These data demonstrate that PCE prevents adverse changes in the ocular surface and tear fluid through inhibition of hyperosmolar stress-induced inflammation, apoptosis, and oxidation, suggesting that PCE may have the potential to preserve eye health.http://www.mdpi.com/2072-6643/10/10/1550Polygonum cuspidatumhyperosmolar stressexorbital lacrimal gland-exciseddry eyehuman corneal epithelial cellsinflammationapoptosisoxidative stressmatrix metallopeptidase 9 (MMP9)Mucin4 |
| spellingShingle | Bongkyun Park Ik Soo Lee Soo-Wang Hyun Kyuhyung Jo Tae Gu Lee Jin Sook Kim Chan-Sik Kim The Protective Effect of Polygonum cuspidatum (PCE) Aqueous Extract in a Dry Eye Model Nutrients Polygonum cuspidatum hyperosmolar stress exorbital lacrimal gland-excised dry eye human corneal epithelial cells inflammation apoptosis oxidative stress matrix metallopeptidase 9 (MMP9) Mucin4 |
| title | The Protective Effect of Polygonum cuspidatum (PCE) Aqueous Extract in a Dry Eye Model |
| title_full | The Protective Effect of Polygonum cuspidatum (PCE) Aqueous Extract in a Dry Eye Model |
| title_fullStr | The Protective Effect of Polygonum cuspidatum (PCE) Aqueous Extract in a Dry Eye Model |
| title_full_unstemmed | The Protective Effect of Polygonum cuspidatum (PCE) Aqueous Extract in a Dry Eye Model |
| title_short | The Protective Effect of Polygonum cuspidatum (PCE) Aqueous Extract in a Dry Eye Model |
| title_sort | protective effect of polygonum cuspidatum pce aqueous extract in a dry eye model |
| topic | Polygonum cuspidatum hyperosmolar stress exorbital lacrimal gland-excised dry eye human corneal epithelial cells inflammation apoptosis oxidative stress matrix metallopeptidase 9 (MMP9) Mucin4 |
| url | http://www.mdpi.com/2072-6643/10/10/1550 |
| work_keys_str_mv | AT bongkyunpark theprotectiveeffectofpolygonumcuspidatumpceaqueousextractinadryeyemodel AT iksoolee theprotectiveeffectofpolygonumcuspidatumpceaqueousextractinadryeyemodel AT soowanghyun theprotectiveeffectofpolygonumcuspidatumpceaqueousextractinadryeyemodel AT kyuhyungjo theprotectiveeffectofpolygonumcuspidatumpceaqueousextractinadryeyemodel AT taegulee theprotectiveeffectofpolygonumcuspidatumpceaqueousextractinadryeyemodel AT jinsookkim theprotectiveeffectofpolygonumcuspidatumpceaqueousextractinadryeyemodel AT chansikkim theprotectiveeffectofpolygonumcuspidatumpceaqueousextractinadryeyemodel AT bongkyunpark protectiveeffectofpolygonumcuspidatumpceaqueousextractinadryeyemodel AT iksoolee protectiveeffectofpolygonumcuspidatumpceaqueousextractinadryeyemodel AT soowanghyun protectiveeffectofpolygonumcuspidatumpceaqueousextractinadryeyemodel AT kyuhyungjo protectiveeffectofpolygonumcuspidatumpceaqueousextractinadryeyemodel AT taegulee protectiveeffectofpolygonumcuspidatumpceaqueousextractinadryeyemodel AT jinsookkim protectiveeffectofpolygonumcuspidatumpceaqueousextractinadryeyemodel AT chansikkim protectiveeffectofpolygonumcuspidatumpceaqueousextractinadryeyemodel |