Detection of Recombinant Proteins SOX2 and OCT4 Interacting in HEK293T Cells Using Real-Time Quantitative PCR

Detection of Recombinant Proteins SOX2 and OCT4 Interacting in HEK293T Cells Using Real-Time Quantitative PCR

In vivo biotinylation using wild-type and mutants of biotin ligases is now widely applied for the study of cellular proteomes. The commercial availability of kits for the highly efficient purification of biotinylated proteins and their excellent compatibility with LC-MS/MS protocols are the main rea...

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Bibliographic Details
Main Authors: Darkhan Kanayev, Diana Abilmazhenova, Ilyas Akhmetollayev, Aliya Sekenova, Vyacheslav Ogay, Arman Kulyyassov
Format: Article
Language:English
Published: MDPI AG 2022-12-01
Series:Life
Subjects:
biotin ligases
Biotin Acceptor Peptide (BAP)
liquid chromatography tandem mass-spectrometry (LC-MS/MS)
Proximity Utilizing Biotinylation (PUB)
reverse transcription polymerase chain reaction (RT-PCR)
transcription factors of pluripotency
Online Access:https://www.mdpi.com/2075-1729/13/1/107

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https://www.mdpi.com/2075-1729/13/1/107

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