Bioactivity and Chemical Profile of <i>Rubus idaeus</i> L. Leaves Steam-Distillation Extract
The leaves of <i>Rubus idaeus</i> L., a by-product of the fruit food industry, are a known source of bioactive molecules, although the chemical composition has only been partially investigated. The main objective of this study was to examine the biological activities and the chemical com...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/11/10/1455 |
| _version_ | 1797499930567245824 |
|---|---|
| author | Diana De Santis Katya Carbone Stefania Garzoli Valentina Laghezza Masci Giovanni Turchetti |
| author_facet | Diana De Santis Katya Carbone Stefania Garzoli Valentina Laghezza Masci Giovanni Turchetti |
| author_sort | Diana De Santis |
| collection | DOAJ |
| description | The leaves of <i>Rubus idaeus</i> L., a by-product of the fruit food industry, are a known source of bioactive molecules, although the chemical composition has only been partially investigated. The main objective of this study was to examine the biological activities and the chemical composition of the extract of leaves of <i>R. idaeus</i> (RH), obtained by steam distillation (SD). The antioxidant capacity; the total phenolic content (TPC); the cytotoxic activity against tumor cell lines; and the antibacterial activity, in addition to the study of the chemical fingerprinting, carried out by Gas/Chromatography-Mass-Spectrometry (GC/MS) and Headspace (HS)-GC/MS, were established. The extract showed a strong antioxidant capacity and a modest antibacterial activity against two bacterial strains, as well as significant cytotoxic activity against tumor cell lines (Caco-2 and HL60) and being proliferative on healthy cells. Many of the GC-identified volatile molecules (1,8-cineol, β-linalool, geraniol, caryophyllene, τ-muurolol, citral, α-terpineol, 3- carene, α-terpinen-7-al, etc.) can explain most of the biological properties exhibited by the extract of <i>R. idaeus</i> L. The high biological activity of the RH and the high compatibility with the various matrices suggest good prospects for this extract, both in the food and cosmetic fields or in dietary supplements for improving human health. |
| first_indexed | 2024-03-10T03:54:32Z |
| format | Article |
| id | doaj.art-d4a2ae1afa494758b46611a8591aa209 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T03:54:32Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-d4a2ae1afa494758b46611a8591aa2092023-11-23T10:59:23ZengMDPI AGFoods2304-81582022-05-011110145510.3390/foods11101455Bioactivity and Chemical Profile of <i>Rubus idaeus</i> L. Leaves Steam-Distillation ExtractDiana De Santis0Katya Carbone1Stefania Garzoli2Valentina Laghezza Masci3Giovanni Turchetti4Department for Innovation in the Biological, Agrofood and Forestry Systems, University of Tuscia Via S. C. de Lellis, 01100 Viterbo, ItalyCREA Research Centre for Olive, Fruit and Citrus Crops, Via di Fioranello 52, 00134 Rome, ItalyDepartment of Drug Chemistry and Technology, Sapienza University, Square Aldo Moro 5, 00185 Rome, ItalyDepartment for Innovation in the Biological, Agrofood and Forestry Systems, University of Tuscia Via S. C. de Lellis, 01100 Viterbo, ItalyDepartment for Innovation in the Biological, Agrofood and Forestry Systems, University of Tuscia Via S. C. de Lellis, 01100 Viterbo, ItalyThe leaves of <i>Rubus idaeus</i> L., a by-product of the fruit food industry, are a known source of bioactive molecules, although the chemical composition has only been partially investigated. The main objective of this study was to examine the biological activities and the chemical composition of the extract of leaves of <i>R. idaeus</i> (RH), obtained by steam distillation (SD). The antioxidant capacity; the total phenolic content (TPC); the cytotoxic activity against tumor cell lines; and the antibacterial activity, in addition to the study of the chemical fingerprinting, carried out by Gas/Chromatography-Mass-Spectrometry (GC/MS) and Headspace (HS)-GC/MS, were established. The extract showed a strong antioxidant capacity and a modest antibacterial activity against two bacterial strains, as well as significant cytotoxic activity against tumor cell lines (Caco-2 and HL60) and being proliferative on healthy cells. Many of the GC-identified volatile molecules (1,8-cineol, β-linalool, geraniol, caryophyllene, τ-muurolol, citral, α-terpineol, 3- carene, α-terpinen-7-al, etc.) can explain most of the biological properties exhibited by the extract of <i>R. idaeus</i> L. The high biological activity of the RH and the high compatibility with the various matrices suggest good prospects for this extract, both in the food and cosmetic fields or in dietary supplements for improving human health.https://www.mdpi.com/2304-8158/11/10/1455raspberry leavessteam-distillationantibacterial activityradical scavenging capacityGC/MS profile |
| spellingShingle | Diana De Santis Katya Carbone Stefania Garzoli Valentina Laghezza Masci Giovanni Turchetti Bioactivity and Chemical Profile of <i>Rubus idaeus</i> L. Leaves Steam-Distillation Extract Foods raspberry leaves steam-distillation antibacterial activity radical scavenging capacity GC/MS profile |
| title | Bioactivity and Chemical Profile of <i>Rubus idaeus</i> L. Leaves Steam-Distillation Extract |
| title_full | Bioactivity and Chemical Profile of <i>Rubus idaeus</i> L. Leaves Steam-Distillation Extract |
| title_fullStr | Bioactivity and Chemical Profile of <i>Rubus idaeus</i> L. Leaves Steam-Distillation Extract |
| title_full_unstemmed | Bioactivity and Chemical Profile of <i>Rubus idaeus</i> L. Leaves Steam-Distillation Extract |
| title_short | Bioactivity and Chemical Profile of <i>Rubus idaeus</i> L. Leaves Steam-Distillation Extract |
| title_sort | bioactivity and chemical profile of i rubus idaeus i l leaves steam distillation extract |
| topic | raspberry leaves steam-distillation antibacterial activity radical scavenging capacity GC/MS profile |
| url | https://www.mdpi.com/2304-8158/11/10/1455 |
| work_keys_str_mv | AT dianadesantis bioactivityandchemicalprofileofirubusidaeusilleavessteamdistillationextract AT katyacarbone bioactivityandchemicalprofileofirubusidaeusilleavessteamdistillationextract AT stefaniagarzoli bioactivityandchemicalprofileofirubusidaeusilleavessteamdistillationextract AT valentinalaghezzamasci bioactivityandchemicalprofileofirubusidaeusilleavessteamdistillationextract AT giovanniturchetti bioactivityandchemicalprofileofirubusidaeusilleavessteamdistillationextract |