Improving the Biocontrol Potential of Endophytic Bacteria <i>Bacillus subtilis</i> with Salicylic Acid against <i>Phytophthora infestans</i>-Caused Postharvest Potato Tuber Late Blight and Impact on Stored Tubers Quality
Potato (<i>Solanum tuberosum</i> L.) tubers are a highly important food crop in many countries due to their nutritional value and health-promoting properties. Postharvest disease caused by <i>Phytophthora infestans</i> leads to the significant decay of stored potatoes. The ma...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/2/117 |
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| author | Oksana Lastochkina Liudmila Pusenkova Darya Garshina Cemal Kasnak Recep Palamutoglu Irina Shpirnaya Il’dar Mardanshin Igor Maksimov |
| author_facet | Oksana Lastochkina Liudmila Pusenkova Darya Garshina Cemal Kasnak Recep Palamutoglu Irina Shpirnaya Il’dar Mardanshin Igor Maksimov |
| author_sort | Oksana Lastochkina |
| collection | DOAJ |
| description | Potato (<i>Solanum tuberosum</i> L.) tubers are a highly important food crop in many countries due to their nutritional value and health-promoting properties. Postharvest disease caused by <i>Phytophthora infestans</i> leads to the significant decay of stored potatoes. The main objective of this study was to evaluate the effects of the endophytic bacteria, <i>Bacillus subtilis</i> (strain 10–4), or its combination with salicylic acid (SA), on some resistance and quality traits of stored <i>Ph. infestans</i>-infected potato tubers. The experiments were conducted using hydroponically grown potato mini-tubers, infected prior to storage with <i>Ph. infestans</i>, and then coated with <i>B. subtilis</i>, alone and in combination with SA, which were then stored for six months. The results revealed that infection with <i>Ph. infestans</i> significantly increased tuber late blight incidence (up to 90–100%) and oxidative and osmotic damage (i.e., malondialdehyde and proline) in tubers. These phenomena were accompanied by a decrease in starch, reducing sugars (RS), and total dry matter (TDM) contents and an increase in amylase (AMY) activity. Moreover, total glycoalkaloids (GA) (α-solanine, α-chaconine) notably increased in infected tubers, exceeding (by 1.6 times) permissible safe levels (200 mg/kg FW). Treatments with <i>B. subtilis</i> or its combination with SA decreased <i>Ph. infestans</i>-activated tuber late blight incidence (by 30–40%) and reduced oxidative and osmotic damages (i.e., malondialdehyde and proline) and AMY activity in stored, infected tubers. Additionally, these treatments decreased pathogen-activated GA accumulation and increased ascorbic acid in stored tubers. Thus, the results indicated that endophytic bacteria <i>B. subtilis</i>, individually, and especially in combination with SA, have the potential to increase potato postharvest resistance to late blight and improve tuber quality in long-term storage. |
| first_indexed | 2024-03-09T21:48:24Z |
| format | Article |
| id | doaj.art-d4a8de95f3734f90b7d9975cd09a4563 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T21:48:24Z |
| publishDate | 2022-01-01 |
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| series | Horticulturae |
| spelling | doaj.art-d4a8de95f3734f90b7d9975cd09a45632023-11-23T20:11:57ZengMDPI AGHorticulturae2311-75242022-01-018211710.3390/horticulturae8020117Improving the Biocontrol Potential of Endophytic Bacteria <i>Bacillus subtilis</i> with Salicylic Acid against <i>Phytophthora infestans</i>-Caused Postharvest Potato Tuber Late Blight and Impact on Stored Tubers QualityOksana Lastochkina0Liudmila Pusenkova1Darya Garshina2Cemal Kasnak3Recep Palamutoglu4Irina Shpirnaya5Il’dar Mardanshin6Igor Maksimov7Institute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, 450054 Ufa, RussiaBashkir Research Institute of Agriculture—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, 450059 Ufa, RussiaBashkir Research Institute of Agriculture—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, 450059 Ufa, RussiaDepartment of Nutrition and Dietetics, Faculty of Health Sciences, Afyonkarahisar Health Sciences University, Afyon 2078, TurkeyDepartment of Nutrition and Dietetics, Faculty of Health Sciences, Afyonkarahisar Health Sciences University, Afyon 2078, TurkeyDepartment of Biology, Bashkir State University, 450076 Ufa, RussiaBashkir Research Institute of Agriculture—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, 450059 Ufa, RussiaInstitute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, 450054 Ufa, RussiaPotato (<i>Solanum tuberosum</i> L.) tubers are a highly important food crop in many countries due to their nutritional value and health-promoting properties. Postharvest disease caused by <i>Phytophthora infestans</i> leads to the significant decay of stored potatoes. The main objective of this study was to evaluate the effects of the endophytic bacteria, <i>Bacillus subtilis</i> (strain 10–4), or its combination with salicylic acid (SA), on some resistance and quality traits of stored <i>Ph. infestans</i>-infected potato tubers. The experiments were conducted using hydroponically grown potato mini-tubers, infected prior to storage with <i>Ph. infestans</i>, and then coated with <i>B. subtilis</i>, alone and in combination with SA, which were then stored for six months. The results revealed that infection with <i>Ph. infestans</i> significantly increased tuber late blight incidence (up to 90–100%) and oxidative and osmotic damage (i.e., malondialdehyde and proline) in tubers. These phenomena were accompanied by a decrease in starch, reducing sugars (RS), and total dry matter (TDM) contents and an increase in amylase (AMY) activity. Moreover, total glycoalkaloids (GA) (α-solanine, α-chaconine) notably increased in infected tubers, exceeding (by 1.6 times) permissible safe levels (200 mg/kg FW). Treatments with <i>B. subtilis</i> or its combination with SA decreased <i>Ph. infestans</i>-activated tuber late blight incidence (by 30–40%) and reduced oxidative and osmotic damages (i.e., malondialdehyde and proline) and AMY activity in stored, infected tubers. Additionally, these treatments decreased pathogen-activated GA accumulation and increased ascorbic acid in stored tubers. Thus, the results indicated that endophytic bacteria <i>B. subtilis</i>, individually, and especially in combination with SA, have the potential to increase potato postharvest resistance to late blight and improve tuber quality in long-term storage.https://www.mdpi.com/2311-7524/8/2/117endophytic bacteria <i>Bacillus subtilis</i><i>Phytophthora infestans</i>salicylic acidpotatostoragepostharvest decay |
| spellingShingle | Oksana Lastochkina Liudmila Pusenkova Darya Garshina Cemal Kasnak Recep Palamutoglu Irina Shpirnaya Il’dar Mardanshin Igor Maksimov Improving the Biocontrol Potential of Endophytic Bacteria <i>Bacillus subtilis</i> with Salicylic Acid against <i>Phytophthora infestans</i>-Caused Postharvest Potato Tuber Late Blight and Impact on Stored Tubers Quality Horticulturae endophytic bacteria <i>Bacillus subtilis</i> <i>Phytophthora infestans</i> salicylic acid potato storage postharvest decay |
| title | Improving the Biocontrol Potential of Endophytic Bacteria <i>Bacillus subtilis</i> with Salicylic Acid against <i>Phytophthora infestans</i>-Caused Postharvest Potato Tuber Late Blight and Impact on Stored Tubers Quality |
| title_full | Improving the Biocontrol Potential of Endophytic Bacteria <i>Bacillus subtilis</i> with Salicylic Acid against <i>Phytophthora infestans</i>-Caused Postharvest Potato Tuber Late Blight and Impact on Stored Tubers Quality |
| title_fullStr | Improving the Biocontrol Potential of Endophytic Bacteria <i>Bacillus subtilis</i> with Salicylic Acid against <i>Phytophthora infestans</i>-Caused Postharvest Potato Tuber Late Blight and Impact on Stored Tubers Quality |
| title_full_unstemmed | Improving the Biocontrol Potential of Endophytic Bacteria <i>Bacillus subtilis</i> with Salicylic Acid against <i>Phytophthora infestans</i>-Caused Postharvest Potato Tuber Late Blight and Impact on Stored Tubers Quality |
| title_short | Improving the Biocontrol Potential of Endophytic Bacteria <i>Bacillus subtilis</i> with Salicylic Acid against <i>Phytophthora infestans</i>-Caused Postharvest Potato Tuber Late Blight and Impact on Stored Tubers Quality |
| title_sort | improving the biocontrol potential of endophytic bacteria i bacillus subtilis i with salicylic acid against i phytophthora infestans i caused postharvest potato tuber late blight and impact on stored tubers quality |
| topic | endophytic bacteria <i>Bacillus subtilis</i> <i>Phytophthora infestans</i> salicylic acid potato storage postharvest decay |
| url | https://www.mdpi.com/2311-7524/8/2/117 |
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