Molecular Cloning and Production of Recombinant Phytase from Bacillus subtilis ASUIA243 in Pichia pastoris

Molecular Cloning and Production of Recombinant Phytase from Bacillus subtilis ASUIA243 in Pichia pastoris

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Phytase gene obtained from Bacillus subtilis ASUIA243 was cloned into a medium vector and transformed into E. coli. Restriction enzyme digestion was conducted to get blunt-ended phytase gene and ligated into the Pichia expression vector, pPICZαA. The recombinant vector, pPICZαA-243HPp was then linea...

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Bibliographic Details
Main Authors: Nor Soleha Mohd Dali, Tamrin Nuge, Mohd Hafidz Mahamad Maifiah, Faridah Yusof, Anis Shobirin Meor Hussin, Abd-Elaziem Farouk, and Hamzah Mohd. Salleh
Format: Article
Language:English
Published: IIUM Press, International Islamic University Malaysia 2011-12-01
Series:International Islamic University Malaysia Engineering Journal
Online Access:http://journals.iium.edu.my/ejournal/index.php/iiumej/article/view/211

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http://journals.iium.edu.my/ejournal/index.php/iiumej/article/view/211

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