Preliminary studies on transgenic human IFN-γ gene in tobacco(转人γIFN基因烟草的初步研究)
以TA克隆法构建中间载体PUCF1,再构建γ干扰素基因表达载体PBIF1。通过农杆菌转化导入烟草叶圆片外植体。经4轮梯度卡那霉素(Kanamycin)递增筛选，获得抗性植株。经PCR证实其中部分植株已将人γ干扰素基因片段整合到烟草基因组中，从而为成功地建立烟草植物反应器生产人γ干扰素基因奠定了基础。...
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2000-07-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/zjup/1008-9497.2000.27.4.464-468 |
| _version_ | 1827303685673189376 |
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| author | YANGRong(杨蓉) ZHUMu-yuan(朱睦元) ZHOUQing(周晴) |
| author_facet | YANGRong(杨蓉) ZHUMu-yuan(朱睦元) ZHOUQing(周晴) |
| author_sort | YANGRong(杨蓉) |
| collection | DOAJ |
| description | 以TA克隆法构建中间载体PUCF1,再构建γ干扰素基因表达载体PBIF1。通过农杆菌转化导入烟草叶圆片外植体。经4轮梯度卡那霉素(Kanamycin)递增筛选，获得抗性植株。经PCR证实其中部分植株已将人γ干扰素基因片段整合到烟草基因组中，从而为成功地建立烟草植物反应器生产人γ干扰素基因奠定了基础。 |
| first_indexed | 2024-04-24T17:05:10Z |
| format | Article |
| id | doaj.art-d4be15525e5a4deda547e1bdf8dcf1c2 |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T17:05:10Z |
| publishDate | 2000-07-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-d4be15525e5a4deda547e1bdf8dcf1c22024-03-29T01:58:16ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972000-07-01274464468zjup/1008-9497.2000.27.4.464-468Preliminary studies on transgenic human IFN-γ gene in tobacco(转人γIFN基因烟草的初步研究)YANGRong(杨蓉)0ZHUMu-yuan(朱睦元)1ZHOUQing(周晴)2Life Science College, Zhejiang University, Hangzhou 310027, China(浙江大学生命科学学院，浙江 杭州 310012)Life Science College, Zhejiang University, Hangzhou 310027, China(浙江大学生命科学学院，浙江 杭州 310012)Life Science College, Zhejiang University, Hangzhou 310027, China(浙江大学生命科学学院，浙江 杭州 310012)以TA克隆法构建中间载体PUCF1,再构建γ干扰素基因表达载体PBIF1。通过农杆菌转化导入烟草叶圆片外植体。经4轮梯度卡那霉素(Kanamycin)递增筛选，获得抗性植株。经PCR证实其中部分植株已将人γ干扰素基因片段整合到烟草基因组中，从而为成功地建立烟草植物反应器生产人γ干扰素基因奠定了基础。https://doi.org/zjup/1008-9497.2000.27.4.464-468人γ干扰素基因烟草农杆菌介导转基因植株植物反应器 |
| spellingShingle | YANGRong(杨蓉) ZHUMu-yuan(朱睦元) ZHOUQing(周晴) Preliminary studies on transgenic human IFN-γ gene in tobacco(转人γIFN基因烟草的初步研究) Zhejiang Daxue xuebao. Lixue ban 人γ干扰素基因 烟草 农杆菌介导 转基因植株 植物反应器 |
| title | Preliminary studies on transgenic human IFN-γ gene in tobacco(转人γIFN基因烟草的初步研究) |
| title_full | Preliminary studies on transgenic human IFN-γ gene in tobacco(转人γIFN基因烟草的初步研究) |
| title_fullStr | Preliminary studies on transgenic human IFN-γ gene in tobacco(转人γIFN基因烟草的初步研究) |
| title_full_unstemmed | Preliminary studies on transgenic human IFN-γ gene in tobacco(转人γIFN基因烟草的初步研究) |
| title_short | Preliminary studies on transgenic human IFN-γ gene in tobacco(转人γIFN基因烟草的初步研究) |
| title_sort | preliminary studies on transgenic human ifn γ gene in tobacco 转人γifn基因烟草的初步研究 |
| topic | 人γ干扰素基因 烟草 农杆菌介导 转基因植株 植物反应器 |
| url | https://doi.org/zjup/1008-9497.2000.27.4.464-468 |
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