A Versatile Assay for the Identification of RNA Silencing Suppressors Based on Complementation of Viral Movement
The cell-to-cell movement of Turnip crinkle virus (TCV) in Nicotiana benthamiana requires the presence of its coat protein (CP), a known suppressor of RNA silencing. RNA transcripts of a TCV construct containing a reporter gene (green fluorescent protein) (TCV-sGFP) in place of the CP open reading f...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2008-07-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-7-0879 |