Drug resistance analysis by next generation sequencing in Leishmania
The use of next generation sequencing has the power to expedite the identification of drug resistance determinants and biomarkers and was applied successfully to drug resistance studies in Leishmania. This allowed the identification of modulation in gene expression, gene dosage alterations, changes...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015-04-01
|
| Series: | International Journal for Parasitology: Drugs and Drug Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211320714000281 |
| _version_ | 1818254263767269376 |
|---|---|
| author | Philippe Leprohon Christopher Fernandez-Prada Élodie Gazanion Rubens Monte-Neto Marc Ouellette |
| author_facet | Philippe Leprohon Christopher Fernandez-Prada Élodie Gazanion Rubens Monte-Neto Marc Ouellette |
| author_sort | Philippe Leprohon |
| collection | DOAJ |
| description | The use of next generation sequencing has the power to expedite the identification of drug resistance determinants and biomarkers and was applied successfully to drug resistance studies in Leishmania. This allowed the identification of modulation in gene expression, gene dosage alterations, changes in chromosome copy numbers and single nucleotide polymorphisms that correlated with resistance in Leishmania strains derived from the laboratory and from the field. An impressive heterogeneity at the population level was also observed, individual clones within populations often differing in both genotypes and phenotypes, hence complicating the elucidation of resistance mechanisms. This review summarizes the most recent highlights that whole genome sequencing brought to our understanding of Leishmania drug resistance and likely new directions. |
| first_indexed | 2024-12-12T16:53:12Z |
| format | Article |
| id | doaj.art-d4d2b33ba1604b8fac5d92b27ced955f |
| institution | Directory Open Access Journal |
| issn | 2211-3207 |
| language | English |
| last_indexed | 2024-12-12T16:53:12Z |
| publishDate | 2015-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal for Parasitology: Drugs and Drug Resistance |
| spelling | doaj.art-d4d2b33ba1604b8fac5d92b27ced955f2022-12-22T00:18:18ZengElsevierInternational Journal for Parasitology: Drugs and Drug Resistance2211-32072015-04-0151263510.1016/j.ijpddr.2014.09.005Drug resistance analysis by next generation sequencing in LeishmaniaPhilippe LeprohonChristopher Fernandez-PradaÉlodie GazanionRubens Monte-NetoMarc OuelletteThe use of next generation sequencing has the power to expedite the identification of drug resistance determinants and biomarkers and was applied successfully to drug resistance studies in Leishmania. This allowed the identification of modulation in gene expression, gene dosage alterations, changes in chromosome copy numbers and single nucleotide polymorphisms that correlated with resistance in Leishmania strains derived from the laboratory and from the field. An impressive heterogeneity at the population level was also observed, individual clones within populations often differing in both genotypes and phenotypes, hence complicating the elucidation of resistance mechanisms. This review summarizes the most recent highlights that whole genome sequencing brought to our understanding of Leishmania drug resistance and likely new directions.http://www.sciencedirect.com/science/article/pii/S2211320714000281LeishmaniaGenomeNext generation sequencingDrug resistanceAneuploidySingle nucleotide polymorphismsGene amplification |
| spellingShingle | Philippe Leprohon Christopher Fernandez-Prada Élodie Gazanion Rubens Monte-Neto Marc Ouellette Drug resistance analysis by next generation sequencing in Leishmania International Journal for Parasitology: Drugs and Drug Resistance Leishmania Genome Next generation sequencing Drug resistance Aneuploidy Single nucleotide polymorphisms Gene amplification |
| title | Drug resistance analysis by next generation sequencing in Leishmania |
| title_full | Drug resistance analysis by next generation sequencing in Leishmania |
| title_fullStr | Drug resistance analysis by next generation sequencing in Leishmania |
| title_full_unstemmed | Drug resistance analysis by next generation sequencing in Leishmania |
| title_short | Drug resistance analysis by next generation sequencing in Leishmania |
| title_sort | drug resistance analysis by next generation sequencing in leishmania |
| topic | Leishmania Genome Next generation sequencing Drug resistance Aneuploidy Single nucleotide polymorphisms Gene amplification |
| url | http://www.sciencedirect.com/science/article/pii/S2211320714000281 |
| work_keys_str_mv | AT philippeleprohon drugresistanceanalysisbynextgenerationsequencinginleishmania AT christopherfernandezprada drugresistanceanalysisbynextgenerationsequencinginleishmania AT elodiegazanion drugresistanceanalysisbynextgenerationsequencinginleishmania AT rubensmonteneto drugresistanceanalysisbynextgenerationsequencinginleishmania AT marcouellette drugresistanceanalysisbynextgenerationsequencinginleishmania |