CRISPR/Cas9-based targeting of fluorescent reporters to human iPSCs to isolate atrial and ventricular-specific cardiomyocytes

CRISPR/Cas9-based targeting of fluorescent reporters to human iPSCs to isolate atrial and ventricular-specific cardiomyocytes

Abstract Generating cardiomyocytes (CMs) from human induced pluripotent stem cells (hiPSCs) has represented a significant advance in our ability to model cardiac disease. Current differentiation protocols, however, have limited use due to their production of heterogenous cell populations, primarily...

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Bibliographic Details
Main Authors: Orlando Chirikian, William R. Goodyer, Elda Dzilic, Vahid Serpooshan, Jan W. Buikema, Wesley McKeithan, HaoDi Wu, Guang Li, Soah Lee, Markus Merk, Francisco Galdos, Aimee Beck, Alexandre J. S. Ribeiro, Sharon Paige, Mark Mercola, Joseph C. Wu, Beth L. Pruitt, Sean M. Wu
Format: Article
Language:English
Published: Nature Portfolio 2021-02-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-81860-x

Internet

https://doi.org/10.1038/s41598-021-81860-x

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