Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli

Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli

Abstract Background The exploitation of the CRISPR/Cas9 machinery coupled to lambda (λ) recombinase-mediated homologous recombination (recombineering) is becoming the method of choice for genome editing in E. coli. First proposed by Jiang and co-workers, the strategy has been subsequently fine-tuned...

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Bibliographic Details
Main Authors: Francesca Zerbini, Ilaria Zanella, Davide Fraccascia, Enrico König, Carmela Irene, Luca F. Frattini, Michele Tomasi, Laura Fantappiè, Luisa Ganfini, Elena Caproni, Matteo Parri, Alberto Grandi, Guido Grandi
Format: Article
Language:English
Published: BMC 2017-04-01
Series:Microbial Cell Factories
Subjects:
CRISPR-Cas9
High-throughput genome editing
Synthetic biology
Online Access:http://link.springer.com/article/10.1186/s12934-017-0681-1

Internet

http://link.springer.com/article/10.1186/s12934-017-0681-1

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