Effect of Sample Preparation on the Detection and Quantification of Selected Nuts Allergenic Proteins by LC-MS/MS

Effect of Sample Preparation on the Detection and Quantification of Selected Nuts Allergenic Proteins by LC-MS/MS

The detection and quantification of nut allergens remains a major challenge. The liquid chroma-tography tandem mass spectrometry (LC-MS/MS) is emerging as one of the most widely used methods, but sample preparation prior to the analysis is still a key issue. The objective of this work was to establi...

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Bibliographic Details
Main Authors: Sorel Tchewonpi Sagu, Gerd Huschek, Thomas Homann, Harshadrai M. Rawel
Format: Article
Language:English
Published: MDPI AG 2021-08-01
Series:Molecules
Subjects:
nut allergenic proteins
sample preparation
protein extraction
tryptic digestion
microwave assisted digestion
SDS PAGE
Online Access:https://www.mdpi.com/1420-3049/26/15/4698

Internet

https://www.mdpi.com/1420-3049/26/15/4698

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