Neuroprotective Effects of Shenqi Fuzheng Injection in a Transgenic SOD1-G93A Mouse Model of Amyotrophic Lateral Sclerosis
Background: Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease, in the pathogenesis of which oxidative stress (OS) was believed to play a key role. Shenqi Fuzheng Injection (SFI) concocted from two kinds of Chinese medicinal herbs, Radix Codonopsis and Radix Astragali, wa...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-10-01
|
| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.701886/full |
| _version_ | 1830442866490474496 |
|---|---|
| author | Kazuo Sugimoto Kazuo Sugimoto Jia Liu Jia Liu MingXuan Li YueBo Song Chi Zhang Chi Zhang ZhiGuang Zhai ZhiGuang Zhai Ying Gao Ying Gao |
| author_facet | Kazuo Sugimoto Kazuo Sugimoto Jia Liu Jia Liu MingXuan Li YueBo Song Chi Zhang Chi Zhang ZhiGuang Zhai ZhiGuang Zhai Ying Gao Ying Gao |
| author_sort | Kazuo Sugimoto |
| collection | DOAJ |
| description | Background: Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease, in the pathogenesis of which oxidative stress (OS) was believed to play a key role. Shenqi Fuzheng Injection (SFI) concocted from two kinds of Chinese medicinal herbs, Radix Codonopsis and Radix Astragali, was proven to be eligible to reduce the OS injury and increase the activity of the nuclear factor-erythroid-2–related factor 2 (Nrf2) pathway, an antioxidant enzymes inducer.Objective: We aim to investigate the effects and potential mechanisms underlying the action of SFI on a well-established transgenic mouse model of ALS.Methods: Transgenic SOD1-G93A mice were intraperitoneally injected with SFI (40 ml/kg) three times a week from 87 days of age. Motor function, survival, pathological manifestations in the brain, and Nrf2 pathway-related assessments of the mice were performed.Results: SFI treatment efficiently postponed the disease onset (p = 0.022) and extended the overall survival (p = 0.038) of the SOD1-G93A mice. Moreover, SFI significantly reduced motor neuron loss (p < 0.001) and astrocytic activation (p < 0.05) in the motor cortex of the brain of SOD1-G93A mice at 130 days of age. The protective effects of SFI in the SOD1-G93A mice were associated with decreasing the level of malondialdehyde (p < 0.05) and increasing the levels of superoxide dismutase (p < 0.05), Nrf2 (p < 0.05), heme oxygenase-1 (p < 0.05), and glutathione S-transferase (p < 0.05) in the SOD1-G93A mice.Conclusion: The SFI treatment efficiently extended the overall survival and improved the pathological manifestations of the brain via alleviating the OS injury and activating the Nrf2 pathway in the animal model of ALS, which made SFI a potentially promising candidate for ALS treatment. |
| first_indexed | 2024-12-21T05:46:12Z |
| format | Article |
| id | doaj.art-d547034609764b12bd21b45b7d616511 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-21T05:46:12Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-d547034609764b12bd21b45b7d6165112022-12-21T19:14:08ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-10-011210.3389/fphar.2021.701886701886Neuroprotective Effects of Shenqi Fuzheng Injection in a Transgenic SOD1-G93A Mouse Model of Amyotrophic Lateral SclerosisKazuo Sugimoto0Kazuo Sugimoto1Jia Liu2Jia Liu3MingXuan Li4YueBo Song5Chi Zhang6Chi Zhang7ZhiGuang Zhai8ZhiGuang Zhai9Ying Gao10Ying Gao11Department of Neurology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, ChinaInstitute for Brain Disorders, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Neurology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, ChinaInstitute for Brain Disorders, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Neurology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Neurology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Neurology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, ChinaInstitute for Brain Disorders, Beijing University of Chinese Medicine, Beijing, ChinaInstitute of Basic Theory for Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, ChinaBeijing University of Chinese Medicine Third Affiliated Hospital, Beijing, ChinaDepartment of Neurology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, ChinaInstitute for Brain Disorders, Beijing University of Chinese Medicine, Beijing, ChinaBackground: Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease, in the pathogenesis of which oxidative stress (OS) was believed to play a key role. Shenqi Fuzheng Injection (SFI) concocted from two kinds of Chinese medicinal herbs, Radix Codonopsis and Radix Astragali, was proven to be eligible to reduce the OS injury and increase the activity of the nuclear factor-erythroid-2–related factor 2 (Nrf2) pathway, an antioxidant enzymes inducer.Objective: We aim to investigate the effects and potential mechanisms underlying the action of SFI on a well-established transgenic mouse model of ALS.Methods: Transgenic SOD1-G93A mice were intraperitoneally injected with SFI (40 ml/kg) three times a week from 87 days of age. Motor function, survival, pathological manifestations in the brain, and Nrf2 pathway-related assessments of the mice were performed.Results: SFI treatment efficiently postponed the disease onset (p = 0.022) and extended the overall survival (p = 0.038) of the SOD1-G93A mice. Moreover, SFI significantly reduced motor neuron loss (p < 0.001) and astrocytic activation (p < 0.05) in the motor cortex of the brain of SOD1-G93A mice at 130 days of age. The protective effects of SFI in the SOD1-G93A mice were associated with decreasing the level of malondialdehyde (p < 0.05) and increasing the levels of superoxide dismutase (p < 0.05), Nrf2 (p < 0.05), heme oxygenase-1 (p < 0.05), and glutathione S-transferase (p < 0.05) in the SOD1-G93A mice.Conclusion: The SFI treatment efficiently extended the overall survival and improved the pathological manifestations of the brain via alleviating the OS injury and activating the Nrf2 pathway in the animal model of ALS, which made SFI a potentially promising candidate for ALS treatment.https://www.frontiersin.org/articles/10.3389/fphar.2021.701886/fullShenqi Fuzheng Injectionamyotrophic lateral sclerosisneurodegenerationneuroprotective effectoxidative stressNrf2 pathway |
| spellingShingle | Kazuo Sugimoto Kazuo Sugimoto Jia Liu Jia Liu MingXuan Li YueBo Song Chi Zhang Chi Zhang ZhiGuang Zhai ZhiGuang Zhai Ying Gao Ying Gao Neuroprotective Effects of Shenqi Fuzheng Injection in a Transgenic SOD1-G93A Mouse Model of Amyotrophic Lateral Sclerosis Frontiers in Pharmacology Shenqi Fuzheng Injection amyotrophic lateral sclerosis neurodegeneration neuroprotective effect oxidative stress Nrf2 pathway |
| title | Neuroprotective Effects of Shenqi Fuzheng Injection in a Transgenic SOD1-G93A Mouse Model of Amyotrophic Lateral Sclerosis |
| title_full | Neuroprotective Effects of Shenqi Fuzheng Injection in a Transgenic SOD1-G93A Mouse Model of Amyotrophic Lateral Sclerosis |
| title_fullStr | Neuroprotective Effects of Shenqi Fuzheng Injection in a Transgenic SOD1-G93A Mouse Model of Amyotrophic Lateral Sclerosis |
| title_full_unstemmed | Neuroprotective Effects of Shenqi Fuzheng Injection in a Transgenic SOD1-G93A Mouse Model of Amyotrophic Lateral Sclerosis |
| title_short | Neuroprotective Effects of Shenqi Fuzheng Injection in a Transgenic SOD1-G93A Mouse Model of Amyotrophic Lateral Sclerosis |
| title_sort | neuroprotective effects of shenqi fuzheng injection in a transgenic sod1 g93a mouse model of amyotrophic lateral sclerosis |
| topic | Shenqi Fuzheng Injection amyotrophic lateral sclerosis neurodegeneration neuroprotective effect oxidative stress Nrf2 pathway |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2021.701886/full |
| work_keys_str_mv | AT kazuosugimoto neuroprotectiveeffectsofshenqifuzhenginjectioninatransgenicsod1g93amousemodelofamyotrophiclateralsclerosis AT kazuosugimoto neuroprotectiveeffectsofshenqifuzhenginjectioninatransgenicsod1g93amousemodelofamyotrophiclateralsclerosis AT jialiu neuroprotectiveeffectsofshenqifuzhenginjectioninatransgenicsod1g93amousemodelofamyotrophiclateralsclerosis AT jialiu neuroprotectiveeffectsofshenqifuzhenginjectioninatransgenicsod1g93amousemodelofamyotrophiclateralsclerosis AT mingxuanli neuroprotectiveeffectsofshenqifuzhenginjectioninatransgenicsod1g93amousemodelofamyotrophiclateralsclerosis AT yuebosong neuroprotectiveeffectsofshenqifuzhenginjectioninatransgenicsod1g93amousemodelofamyotrophiclateralsclerosis AT chizhang neuroprotectiveeffectsofshenqifuzhenginjectioninatransgenicsod1g93amousemodelofamyotrophiclateralsclerosis AT chizhang neuroprotectiveeffectsofshenqifuzhenginjectioninatransgenicsod1g93amousemodelofamyotrophiclateralsclerosis AT zhiguangzhai neuroprotectiveeffectsofshenqifuzhenginjectioninatransgenicsod1g93amousemodelofamyotrophiclateralsclerosis AT zhiguangzhai neuroprotectiveeffectsofshenqifuzhenginjectioninatransgenicsod1g93amousemodelofamyotrophiclateralsclerosis AT yinggao neuroprotectiveeffectsofshenqifuzhenginjectioninatransgenicsod1g93amousemodelofamyotrophiclateralsclerosis AT yinggao neuroprotectiveeffectsofshenqifuzhenginjectioninatransgenicsod1g93amousemodelofamyotrophiclateralsclerosis |