Magnetic Cation Exchange Isolation of Lysozyme from Native Hen Egg White
Two magnetic macroporous cellulose cation exchangers (Iontosorb MG CM 100 and Iontosorb MG SHP 100) were used for one-step isolation of lysozyme from native, undiluted hen egg white. Highly purified lysozyme (purity >96 %) with specific activity similar to that of commercial lysozyme preparations...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Zagreb Faculty of Food Technology and Biotechnology
2007-01-01
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| Series: | Food Technology and Biotechnology |
| Subjects: | |
| Online Access: | http://hrcak.srce.hr/file/36385 |
| Summary: | Two magnetic macroporous cellulose cation exchangers (Iontosorb MG CM 100 and Iontosorb MG SHP 100) were used for one-step isolation of lysozyme from native, undiluted hen egg white. Highly purified lysozyme (purity >96 %) with specific activity similar to that of commercial lysozyme preparations was obtained in both cases. Carboxymethyl-based cation exchanger exhibited substantially higher capacity for lysozyme; maximum adsorption capacity was 138 mg/mL. The lysozyme-depleted egg white can be used in the same way as the routinely used egg white because no dilution of this material was necessary during the purification process. |
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| ISSN: | 1330-9862 1334-2606 |