Establishment and phenotyping of neurosphere cultures from primary neuroblastoma samples [version 1; peer review: 3 approved]

Background: Primary cell culture using serum free media supplemented with growth factors has been used in a number of cancers to propagate primary cells with stem like properties, which form as spherical cellular aggregates. Methods: We systematically evaluated the capacity of freshly disaggregated...

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Bibliographic Details
Main Authors: Jack Barton, Katherine Pacey, Neha Jain, Tessa Kasia, Darren Edwards, Christine Thevanesan, Karin Straathof, Giuseppe Barone, John Anderson
Format: Article
Language:English
Published: F1000 Research Ltd 2019-06-01
Series:F1000Research
Online Access:https://f1000research.com/articles/8-823/v1