Plasmid Curing and Exchange Using a Novel Counter-Selectable Marker Based on Unnatural Amino Acid Incorporation at a Sense Codon
A protocol was designed for plasmid curing using a novel counter-selectable marker, named <i>pylS<sup>ZK</sup>-pylT</i>, in <i>Escherichia coli</i>. The <i>pylS<sup>ZK</sup>-pylT</i> marker consists of the archaeal pyrrolysyl-tRNA synthetas...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/21/11482 |