Bacterial and Eukaryotic Small-Subunit Amplicon Data Do Not Provide a Quantitative Picture of Microbial Communities, but They Are Reliable in the Context of Ecological Interpretations

Bacterial and Eukaryotic Small-Subunit Amplicon Data Do Not Provide a Quantitative Picture of Microbial Communities, but They Are Reliable in the Context of Ecological Interpretations

ABSTRACT High-throughput sequencing (HTS) of gene amplicons is a preferred method of assessing microbial community composition, because it rapidly provides information from a large number of samples at high taxonomic resolution and low costs. However, mock community studies show that HTS data poorly...

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Bibliographic Details
Main Authors: Kasia Piwosz, Tanja Shabarova, Jakob Pernthaler, Thomas Posch, Karel Šimek, Petr Porcal, Michaela M. Salcher
Format: Article
Language:English
Published: American Society for Microbiology 2020-04-01
Series:mSphere
Subjects:
CARD-FISH
amplicon sequencing
bacterial communities
bacterial community structure
bacterial dynamics
eukaryotic communities
Online Access:https://journals.asm.org/doi/10.1128/mSphere.00052-20

Internet

https://journals.asm.org/doi/10.1128/mSphere.00052-20

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