Bacterial and Eukaryotic Small-Subunit Amplicon Data Do Not Provide a Quantitative Picture of Microbial Communities, but They Are Reliable in the Context of Ecological Interpretations
ABSTRACT High-throughput sequencing (HTS) of gene amplicons is a preferred method of assessing microbial community composition, because it rapidly provides information from a large number of samples at high taxonomic resolution and low costs. However, mock community studies show that HTS data poorly...
Main Authors: | Kasia Piwosz, Tanja Shabarova, Jakob Pernthaler, Thomas Posch, Karel Šimek, Petr Porcal, Michaela M. Salcher |
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Format: | Article |
Language: | English |
Published: |
American Society for Microbiology
2020-04-01
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Series: | mSphere |
Subjects: | |
Online Access: | https://journals.asm.org/doi/10.1128/mSphere.00052-20 |
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