Ethanol Positively Modulates Photosynthetic Traits, Antioxidant Defense and Osmoprotectant Levels to Enhance Drought Acclimatization in Soybean
Drought is a major environmental threat to agricultural productivity and food security across the world. Therefore, addressing the detrimental effects of drought on vital crops like soybean has a significant impact on sustainable food production. Priming plants with organic compounds is now being co...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/3/516 |
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| author | Md. Mezanur Rahman Mohammad Golam Mostofa Ashim Kumar Das Touhidur Rahman Anik Sanjida Sultana Keya S. M. Ahsan Md. Arifur Rahman Khan Minhaz Ahmed Md. Abiar Rahman Md. Motaher Hossain Lam-Son Phan Tran |
| author_facet | Md. Mezanur Rahman Mohammad Golam Mostofa Ashim Kumar Das Touhidur Rahman Anik Sanjida Sultana Keya S. M. Ahsan Md. Arifur Rahman Khan Minhaz Ahmed Md. Abiar Rahman Md. Motaher Hossain Lam-Son Phan Tran |
| author_sort | Md. Mezanur Rahman |
| collection | DOAJ |
| description | Drought is a major environmental threat to agricultural productivity and food security across the world. Therefore, addressing the detrimental effects of drought on vital crops like soybean has a significant impact on sustainable food production. Priming plants with organic compounds is now being considered as a promising technique for alleviating the negative effects of drought on plants. In the current study, we evaluated the protective functions of ethanol in enhancing soybean drought tolerance by examining the phenotype, growth attributes, and several physiological and biochemical mechanisms. Our results showed that foliar application of ethanol (20 mM) to drought-stressed soybean plants increased biomass, leaf area per trifoliate, gas exchange features, water-use-efficiency, photosynthetic pigment contents, and leaf relative water content, all of which contributed to the improved growth performance of soybean under drought circumstances. Drought stress, on the other hand, caused significant accumulation of reactive oxygen species (ROS), such as superoxide and hydrogen peroxide, and malondialdehyde, as well as an increase of electrolyte leakage in the leaves, underpinning the evidence of oxidative stress and membrane damage in soybean plants. By comparison, exogenous ethanol reduced the ROS-induced oxidative burden by boosting the activities of antioxidant enzymes, including peroxidase, catalase, glutathione <i>S</i>-transferase, and ascorbate peroxidase, and the content of total flavonoids in soybean leaves exposed to drought stress. Additionally, ethanol supplementation increased the contents of total soluble sugars and free amino acids in the leaves of drought-exposed plants, implying that ethanol likely employed these compounds for osmotic adjustment in soybean under water-shortage conditions. Together, our findings shed light on the ethanol-mediated protective mechanisms by which soybean plants coordinated different morphophysiological and biochemical responses in order to increase their drought tolerance. |
| first_indexed | 2024-03-09T13:52:28Z |
| format | Article |
| id | doaj.art-d5b09162a96c4c6e9ed3f017a9e2f185 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T13:52:28Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-d5b09162a96c4c6e9ed3f017a9e2f1852023-11-30T20:47:54ZengMDPI AGAntioxidants2076-39212022-03-0111351610.3390/antiox11030516Ethanol Positively Modulates Photosynthetic Traits, Antioxidant Defense and Osmoprotectant Levels to Enhance Drought Acclimatization in SoybeanMd. Mezanur Rahman0Mohammad Golam Mostofa1Ashim Kumar Das2Touhidur Rahman Anik3Sanjida Sultana Keya4S. M. Ahsan5Md. Arifur Rahman Khan6Minhaz Ahmed7Md. Abiar Rahman8Md. Motaher Hossain9Lam-Son Phan Tran10Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409, USAInstitute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409, USADepartment of Agroforestry and Environment, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshPlant Pathology Division, Bangladesh Rice Research Institute, Gazipur 1701, BangladeshInstitute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409, USADepartment of Agriculture, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj 8100, BangladeshDepartment of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshDepartment of Agroforestry and Environment, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshDepartment of Agroforestry and Environment, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshDepartment of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshInstitute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409, USADrought is a major environmental threat to agricultural productivity and food security across the world. Therefore, addressing the detrimental effects of drought on vital crops like soybean has a significant impact on sustainable food production. Priming plants with organic compounds is now being considered as a promising technique for alleviating the negative effects of drought on plants. In the current study, we evaluated the protective functions of ethanol in enhancing soybean drought tolerance by examining the phenotype, growth attributes, and several physiological and biochemical mechanisms. Our results showed that foliar application of ethanol (20 mM) to drought-stressed soybean plants increased biomass, leaf area per trifoliate, gas exchange features, water-use-efficiency, photosynthetic pigment contents, and leaf relative water content, all of which contributed to the improved growth performance of soybean under drought circumstances. Drought stress, on the other hand, caused significant accumulation of reactive oxygen species (ROS), such as superoxide and hydrogen peroxide, and malondialdehyde, as well as an increase of electrolyte leakage in the leaves, underpinning the evidence of oxidative stress and membrane damage in soybean plants. By comparison, exogenous ethanol reduced the ROS-induced oxidative burden by boosting the activities of antioxidant enzymes, including peroxidase, catalase, glutathione <i>S</i>-transferase, and ascorbate peroxidase, and the content of total flavonoids in soybean leaves exposed to drought stress. Additionally, ethanol supplementation increased the contents of total soluble sugars and free amino acids in the leaves of drought-exposed plants, implying that ethanol likely employed these compounds for osmotic adjustment in soybean under water-shortage conditions. Together, our findings shed light on the ethanol-mediated protective mechanisms by which soybean plants coordinated different morphophysiological and biochemical responses in order to increase their drought tolerance.https://www.mdpi.com/2076-3921/11/3/516antioxidant enzymesgas exchange featuresosmotic adjustmentoxidative damagephotosynthesisreactive oxygen species |
| spellingShingle | Md. Mezanur Rahman Mohammad Golam Mostofa Ashim Kumar Das Touhidur Rahman Anik Sanjida Sultana Keya S. M. Ahsan Md. Arifur Rahman Khan Minhaz Ahmed Md. Abiar Rahman Md. Motaher Hossain Lam-Son Phan Tran Ethanol Positively Modulates Photosynthetic Traits, Antioxidant Defense and Osmoprotectant Levels to Enhance Drought Acclimatization in Soybean Antioxidants antioxidant enzymes gas exchange features osmotic adjustment oxidative damage photosynthesis reactive oxygen species |
| title | Ethanol Positively Modulates Photosynthetic Traits, Antioxidant Defense and Osmoprotectant Levels to Enhance Drought Acclimatization in Soybean |
| title_full | Ethanol Positively Modulates Photosynthetic Traits, Antioxidant Defense and Osmoprotectant Levels to Enhance Drought Acclimatization in Soybean |
| title_fullStr | Ethanol Positively Modulates Photosynthetic Traits, Antioxidant Defense and Osmoprotectant Levels to Enhance Drought Acclimatization in Soybean |
| title_full_unstemmed | Ethanol Positively Modulates Photosynthetic Traits, Antioxidant Defense and Osmoprotectant Levels to Enhance Drought Acclimatization in Soybean |
| title_short | Ethanol Positively Modulates Photosynthetic Traits, Antioxidant Defense and Osmoprotectant Levels to Enhance Drought Acclimatization in Soybean |
| title_sort | ethanol positively modulates photosynthetic traits antioxidant defense and osmoprotectant levels to enhance drought acclimatization in soybean |
| topic | antioxidant enzymes gas exchange features osmotic adjustment oxidative damage photosynthesis reactive oxygen species |
| url | https://www.mdpi.com/2076-3921/11/3/516 |
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