Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect

With the help of sepiolite, a unique method for transforming DNA into bacteria, based on the Yoshida effect, has been developed recently. However, we confronted many problems when this newest method was tried. Only a few transformants could be obtained even when 100 ng of plasmid pET15b was used, an...

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Bibliographic Details
Main Authors: Masaharu Seno, Li Fu, Haidong Tan
Format: Article
Language:English
Published: MDPI AG 2010-12-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:http://www.mdpi.com/1422-0067/11/12/4962/