Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect
With the help of sepiolite, a unique method for transforming DNA into bacteria, based on the Yoshida effect, has been developed recently. However, we confronted many problems when this newest method was tried. Only a few transformants could be obtained even when 100 ng of plasmid pET15b was used, an...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2010-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/11/12/4962/ |
| _version_ | 1811252471316611072 |
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| author | Masaharu Seno Li Fu Haidong Tan |
| author_facet | Masaharu Seno Li Fu Haidong Tan |
| author_sort | Masaharu Seno |
| collection | DOAJ |
| description | With the help of sepiolite, a unique method for transforming DNA into bacteria, based on the Yoshida effect, has been developed recently. However, we confronted many problems when this newest method was tried. Only a few transformants could be obtained even when 100 ng of plasmid pET15b was used, and a successful result seemed difficult to repeat. To address this problem, we optimized the operating method and could achieve about 15,000 transformants using the same amount of plasmid, which could match the efficiency gained using the calcium chloride transformation method. Meanwhile, the results could also be reproduced well. In the same way, carbon nanotubes were used to attain more than 15,000 transformants in the same situation. Therefore, the transformation method could be extended to other nanomaterials. Meanwhile, compared with the mechanism previously reported, we verified quite a different principle for the mechanism responsible for such a transformation. In sum, this unique transformation can be developed to become the third widely-used transformation method in laboratories in addition to the chemical method and electroporation. |
| first_indexed | 2024-04-12T16:36:06Z |
| format | Article |
| id | doaj.art-d5b6f40cb9bc408eb61328c09ee03886 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T16:36:06Z |
| publishDate | 2010-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d5b6f40cb9bc408eb61328c09ee038862022-12-22T03:25:00ZengMDPI AGInternational Journal of Molecular Sciences1422-00672010-12-0111124962497210.3390/ijms11124962Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida EffectMasaharu SenoLi FuHaidong TanWith the help of sepiolite, a unique method for transforming DNA into bacteria, based on the Yoshida effect, has been developed recently. However, we confronted many problems when this newest method was tried. Only a few transformants could be obtained even when 100 ng of plasmid pET15b was used, and a successful result seemed difficult to repeat. To address this problem, we optimized the operating method and could achieve about 15,000 transformants using the same amount of plasmid, which could match the efficiency gained using the calcium chloride transformation method. Meanwhile, the results could also be reproduced well. In the same way, carbon nanotubes were used to attain more than 15,000 transformants in the same situation. Therefore, the transformation method could be extended to other nanomaterials. Meanwhile, compared with the mechanism previously reported, we verified quite a different principle for the mechanism responsible for such a transformation. In sum, this unique transformation can be developed to become the third widely-used transformation method in laboratories in addition to the chemical method and electroporation.http://www.mdpi.com/1422-0067/11/12/4962/sepiolitecarbon nanotubeplasmid transformationnanomaterialsYoshida effect |
| spellingShingle | Masaharu Seno Li Fu Haidong Tan Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect International Journal of Molecular Sciences sepiolite carbon nanotube plasmid transformation nanomaterials Yoshida effect |
| title | Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect |
| title_full | Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect |
| title_fullStr | Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect |
| title_full_unstemmed | Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect |
| title_short | Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect |
| title_sort | optimization of bacterial plasmid transformation using nanomaterials based on the yoshida effect |
| topic | sepiolite carbon nanotube plasmid transformation nanomaterials Yoshida effect |
| url | http://www.mdpi.com/1422-0067/11/12/4962/ |
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