Analysis of the Activity of Nuclear Export Signals Using Fluorescent BSA Conjugates

Here we demonstrate that fluorescein-labeled BSA conjugated with a mixture of nuclear import and export signals can be used to evaluate export activity. The method is based on the assumption that the intracellular distribution of the labeled conjugate [nuclear/cytoplasmic (N/C) fluorescent ratio] is...

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Bibliographic Details
Main Authors: Carl M. Feldherr, Debra Akin
Format: Article
Language:English
Published: Taylor & Francis Group 2002-06-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/02326st04