Analysis of the Activity of Nuclear Export Signals Using Fluorescent BSA Conjugates
Here we demonstrate that fluorescein-labeled BSA conjugated with a mixture of nuclear import and export signals can be used to evaluate export activity. The method is based on the assumption that the intracellular distribution of the labeled conjugate [nuclear/cytoplasmic (N/C) fluorescent ratio] is...
| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | English |
| Publicado em: |
Taylor & Francis Group
2002-06-01
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| Colecção: | BioTechniques |
| Acesso em linha: | https://www.future-science.com/doi/10.2144/02326st04 |