Sex-Specific Differences in Cytokine Induction by the Glycolipid Adjuvant 7DW8-5 in Mice
7DW8-5 is a potent glycolipid adjuvant that improves malaria vaccine efficacy in mice by inducing IFN-γ and increasing protective CD8<sup>+</sup> T cell responses. The addition of 7DW8-5 was previously shown to improve the efficacy of a CD8<sup>+</sup> T cell-mediated heterol...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/13/1/8 |
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| author | Felicia N. Watson Caroline J. Duncombe Anya C. Kalata Ethan Conrad Sumana Chakravarty B. Kim Lee Sim Stephen L. Hoffman Moriya Tsuji Melanie J. Shears Sean C. Murphy |
| author_facet | Felicia N. Watson Caroline J. Duncombe Anya C. Kalata Ethan Conrad Sumana Chakravarty B. Kim Lee Sim Stephen L. Hoffman Moriya Tsuji Melanie J. Shears Sean C. Murphy |
| author_sort | Felicia N. Watson |
| collection | DOAJ |
| description | 7DW8-5 is a potent glycolipid adjuvant that improves malaria vaccine efficacy in mice by inducing IFN-γ and increasing protective CD8<sup>+</sup> T cell responses. The addition of 7DW8-5 was previously shown to improve the efficacy of a CD8<sup>+</sup> T cell-mediated heterologous ‘prime-and-trap’ malaria vaccine against <i>Plasmodium yoelii</i> sporozoite challenge in inbred female mice. Here, we report significant differential sex-specific responses to 7DW8-5 in inbred and outbred mice. Male mice express significantly less IFN-γ and IL-4 compared to females following intravenous 7DW8-5 administration. Additionally, unlike in female mice, 7DW8-5 did not improve the vaccine efficacy against sporozoite challenge in prime-and-trap vaccinated male mice. Our findings highlight the importance of including both female and male sexes in experimental adjuvant studies. |
| first_indexed | 2024-03-09T13:28:38Z |
| format | Article |
| id | doaj.art-d5c8442a34024f07a5ad4958a8ce3132 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T13:28:38Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-d5c8442a34024f07a5ad4958a8ce31322023-11-30T21:21:26ZengMDPI AGBiomolecules2218-273X2022-12-01131810.3390/biom13010008Sex-Specific Differences in Cytokine Induction by the Glycolipid Adjuvant 7DW8-5 in MiceFelicia N. Watson0Caroline J. Duncombe1Anya C. Kalata2Ethan Conrad3Sumana Chakravarty4B. Kim Lee Sim5Stephen L. Hoffman6Moriya Tsuji7Melanie J. Shears8Sean C. Murphy9Graduate Program in Pathobiology, Department of Global Health, University of Washington, Seattle, WA 98109, USAGraduate Program in Pathobiology, Department of Global Health, University of Washington, Seattle, WA 98109, USADepartment of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98109, USADepartment of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98109, USASanaria Inc., 9800 Medical Center Drive, Suite A209, Rockville, MD 20850, USASanaria Inc., 9800 Medical Center Drive, Suite A209, Rockville, MD 20850, USASanaria Inc., 9800 Medical Center Drive, Suite A209, Rockville, MD 20850, USAAaron Diamond AIDS Research Center, Division of Infectious Diseases, Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USADepartment of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98109, USAGraduate Program in Pathobiology, Department of Global Health, University of Washington, Seattle, WA 98109, USA7DW8-5 is a potent glycolipid adjuvant that improves malaria vaccine efficacy in mice by inducing IFN-γ and increasing protective CD8<sup>+</sup> T cell responses. The addition of 7DW8-5 was previously shown to improve the efficacy of a CD8<sup>+</sup> T cell-mediated heterologous ‘prime-and-trap’ malaria vaccine against <i>Plasmodium yoelii</i> sporozoite challenge in inbred female mice. Here, we report significant differential sex-specific responses to 7DW8-5 in inbred and outbred mice. Male mice express significantly less IFN-γ and IL-4 compared to females following intravenous 7DW8-5 administration. Additionally, unlike in female mice, 7DW8-5 did not improve the vaccine efficacy against sporozoite challenge in prime-and-trap vaccinated male mice. Our findings highlight the importance of including both female and male sexes in experimental adjuvant studies.https://www.mdpi.com/2218-273X/13/1/87DW8-5adjuvantsex<i>Plasmodium</i>malariavaccine |
| spellingShingle | Felicia N. Watson Caroline J. Duncombe Anya C. Kalata Ethan Conrad Sumana Chakravarty B. Kim Lee Sim Stephen L. Hoffman Moriya Tsuji Melanie J. Shears Sean C. Murphy Sex-Specific Differences in Cytokine Induction by the Glycolipid Adjuvant 7DW8-5 in Mice Biomolecules 7DW8-5 adjuvant sex <i>Plasmodium</i> malaria vaccine |
| title | Sex-Specific Differences in Cytokine Induction by the Glycolipid Adjuvant 7DW8-5 in Mice |
| title_full | Sex-Specific Differences in Cytokine Induction by the Glycolipid Adjuvant 7DW8-5 in Mice |
| title_fullStr | Sex-Specific Differences in Cytokine Induction by the Glycolipid Adjuvant 7DW8-5 in Mice |
| title_full_unstemmed | Sex-Specific Differences in Cytokine Induction by the Glycolipid Adjuvant 7DW8-5 in Mice |
| title_short | Sex-Specific Differences in Cytokine Induction by the Glycolipid Adjuvant 7DW8-5 in Mice |
| title_sort | sex specific differences in cytokine induction by the glycolipid adjuvant 7dw8 5 in mice |
| topic | 7DW8-5 adjuvant sex <i>Plasmodium</i> malaria vaccine |
| url | https://www.mdpi.com/2218-273X/13/1/8 |
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