Optimization of a Protocol for Protein Extraction from Calcified Aortic Valves for Proteomics Applications: Development of a Standard Operating Procedure

Optimization of a Protocol for Protein Extraction from Calcified Aortic Valves for Proteomics Applications: Development of a Standard Operating Procedure

The comprehension of the pathophysiological mechanisms, the identification of druggable targets, and putative biomarkers for aortic valve stenosis can be pursued through holistic approaches such as proteomics. However, tissue homogenization and protein extraction are made difficult by tissue calcifi...

Full description

Bibliographic Details
Main Authors: Fábio Trindade, Ana F. Ferreira, Francisca Saraiva, Diana Martins, Vera M. Mendes, Carla Sousa, Cristina Gavina, Adelino Leite-Moreira, Bruno Manadas, Inês Falcão-Pires, Rui Vitorino
Format: Article
Language:English
Published: MDPI AG 2022-09-01
Series:Proteomes
Subjects:
aortic valve
calcification
homogenization
protein extraction
zirconium dioxide beads
proteomics
Online Access:https://www.mdpi.com/2227-7382/10/3/30

Internet

https://www.mdpi.com/2227-7382/10/3/30

Similar Items