| Summary: | Globally, the spread of antibiotic resistance via chicken meat consumption cause serious public health concerns. With this respect, the current
study aimed to investigate the prevalence of thermophilic Campylobacter species isolated from raw meat chicken samples and their genetic
determinants of resistance to various classes of antibiotics. A total of 540 chicken raw meat samples collected from various supermarkets and
slaughterhouses in Istanbul, Turkey were analyzed according to EN ISO 10272-1:2006 standard procedure. For identification of the genus and
species of the isolates, multiplex PCR assay was held. Minimum inhibitory concentrations of the antimicrobial agents (nalidixic acid, ciprofloxacin,
tetracycline, gentamicin, kanamycin, and erythromycin) were initially determined using the broth microdilution method. In addition, the genetic
determinants of antimicrobial resistance were investigated by PCR assays. In total, 357 (66.1%) Campylobacter isolates were obtained including
268 Campylobacter jejuni and 89 Campylobacter coli. Resistance to quinolones (nalidixic acid and ciprofloxacin) was the most common in all strains
(80.1%), followed by resistance to tetracycline"s (70.3%). The lowest resistance was determined as resistance to kanamycin (4.2%). Gentamicin and
erythromycin resistance was not observed in this study. Only five C. coli isolate (1.4%) was classified as multidrug resistant. On the basis of these
data, execute widely presence of antimicrobial resistance to quinolones and tetracycline"s in C. jejuni and C. coli isolates from chicken raw meat
samples and emphasizes that further multidisciplinary studies and novel strategies in the spirit of "One Health" are needed.
|
|---|