Synthesis and in Vitro Screening of Phenylbipyridinylpyrazole Derivatives as Potential Antiproliferative Agents
A series of phenylbipyridinylpyrazoles was synthesized through the reaction of 2-(4-(2-chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl)acetonitrile (4) with different 6-substituted pyridine-3-ylboronic acids. The final compounds 5a–j were screened at 10 µM against over 60 tumor cel...
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MDPI AG
2015-01-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/20/1/1031 |
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| author | Mohammad M. Al-Sanea Ahmed Elkamhawy Ahmed Zakaria Byung Sun Park Youngjoo Kwon So Ha Lee Sang Woo Lee In Tae Kim |
| author_facet | Mohammad M. Al-Sanea Ahmed Elkamhawy Ahmed Zakaria Byung Sun Park Youngjoo Kwon So Ha Lee Sang Woo Lee In Tae Kim |
| author_sort | Mohammad M. Al-Sanea |
| collection | DOAJ |
| description | A series of phenylbipyridinylpyrazoles was synthesized through the reaction of 2-(4-(2-chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl)acetonitrile (4) with different 6-substituted pyridine-3-ylboronic acids. The final compounds 5a–j were screened at 10 µM against over 60 tumor cell lines at the U.S. National Cancer Institute (NCI). In light of the NCI results, compounds 5c and 5h showed a broad spectrum of activity against NCI cell lines with mean growth of 53% and 58%, respectively. Compound 5e behaved differently as it showed high degree of selectivity and potency by inhibiting 96% of growth of leukemia SR cell line at 10 µM. Standard COMPARE analyses were performed at the GI50 level and the results exhibit high correlation in the form of pairwise correlation coefficient (PCC) of more than 0.6 between three of the current compounds and three standard known anticancer agents. Compound 5e demonstrated high correlation levels with merbarone (NSC S336628) with a PCC value of 0.631. Compound 5h showed a considerably high PCC value of 0.626 with dichloroallyl lawsone, while compound 5i, showed PCC values of 0.601 and 0.604 with both dichloroallyl lawsone and N,N-dibenzyldaunomycin (NSC S268242), respectively. These three standard agents have anticancer activity via two major mechanism of actions, inhibition of topoisomerase II and inhibition of biosynthesis of pyrimidine nucleotides, therefore, compounds 5a–j are promising therapeutic agents for targeting different human malignancies. Prediction of drug-likeness and toxicity of these newly synthesized derivatives were also considered. |
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| last_indexed | 2024-12-12T17:44:50Z |
| publishDate | 2015-01-01 |
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| series | Molecules |
| spelling | doaj.art-d5eafdc315e449ff90b561c067b4c40d2022-12-22T00:16:57ZengMDPI AGMolecules1420-30492015-01-012011031104510.3390/molecules20011031molecules20011031Synthesis and in Vitro Screening of Phenylbipyridinylpyrazole Derivatives as Potential Antiproliferative AgentsMohammad M. Al-Sanea0Ahmed Elkamhawy1Ahmed Zakaria2Byung Sun Park3Youngjoo Kwon4So Ha Lee5Sang Woo Lee6In Tae Kim7Chemical Kinomics Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, KoreaChemical Kinomics Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, KoreaChemical Kinomics Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, KoreaChemical Kinomics Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, KoreaCollege of Pharmacy and Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, KoreaChemical Kinomics Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, KoreaDepartment of Chemistry, Kwangwoon University, Seoul 139-701, KoreaDepartment of Chemistry, Kwangwoon University, Seoul 139-701, KoreaA series of phenylbipyridinylpyrazoles was synthesized through the reaction of 2-(4-(2-chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl)acetonitrile (4) with different 6-substituted pyridine-3-ylboronic acids. The final compounds 5a–j were screened at 10 µM against over 60 tumor cell lines at the U.S. National Cancer Institute (NCI). In light of the NCI results, compounds 5c and 5h showed a broad spectrum of activity against NCI cell lines with mean growth of 53% and 58%, respectively. Compound 5e behaved differently as it showed high degree of selectivity and potency by inhibiting 96% of growth of leukemia SR cell line at 10 µM. Standard COMPARE analyses were performed at the GI50 level and the results exhibit high correlation in the form of pairwise correlation coefficient (PCC) of more than 0.6 between three of the current compounds and three standard known anticancer agents. Compound 5e demonstrated high correlation levels with merbarone (NSC S336628) with a PCC value of 0.631. Compound 5h showed a considerably high PCC value of 0.626 with dichloroallyl lawsone, while compound 5i, showed PCC values of 0.601 and 0.604 with both dichloroallyl lawsone and N,N-dibenzyldaunomycin (NSC S268242), respectively. These three standard agents have anticancer activity via two major mechanism of actions, inhibition of topoisomerase II and inhibition of biosynthesis of pyrimidine nucleotides, therefore, compounds 5a–j are promising therapeutic agents for targeting different human malignancies. Prediction of drug-likeness and toxicity of these newly synthesized derivatives were also considered.http://www.mdpi.com/1420-3049/20/1/1031cancercell linesphenylbipyridinylpyrazoleantiproliferative agentsCOMPARE analysis |
| spellingShingle | Mohammad M. Al-Sanea Ahmed Elkamhawy Ahmed Zakaria Byung Sun Park Youngjoo Kwon So Ha Lee Sang Woo Lee In Tae Kim Synthesis and in Vitro Screening of Phenylbipyridinylpyrazole Derivatives as Potential Antiproliferative Agents Molecules cancer cell lines phenylbipyridinylpyrazole antiproliferative agents COMPARE analysis |
| title | Synthesis and in Vitro Screening of Phenylbipyridinylpyrazole Derivatives as Potential Antiproliferative Agents |
| title_full | Synthesis and in Vitro Screening of Phenylbipyridinylpyrazole Derivatives as Potential Antiproliferative Agents |
| title_fullStr | Synthesis and in Vitro Screening of Phenylbipyridinylpyrazole Derivatives as Potential Antiproliferative Agents |
| title_full_unstemmed | Synthesis and in Vitro Screening of Phenylbipyridinylpyrazole Derivatives as Potential Antiproliferative Agents |
| title_short | Synthesis and in Vitro Screening of Phenylbipyridinylpyrazole Derivatives as Potential Antiproliferative Agents |
| title_sort | synthesis and in vitro screening of phenylbipyridinylpyrazole derivatives as potential antiproliferative agents |
| topic | cancer cell lines phenylbipyridinylpyrazole antiproliferative agents COMPARE analysis |
| url | http://www.mdpi.com/1420-3049/20/1/1031 |
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