Amplicon Sequencing of Single-Copy Protein-Coding Genes Reveals Accurate Diversity for Sequence-Discrete Microbiome Populations

Amplicon Sequencing of Single-Copy Protein-Coding Genes Reveals Accurate Diversity for Sequence-Discrete Microbiome Populations

ABSTRACT An in-depth understanding of microbial function and the division of ecological niches requires accurate delineation and identification of microbes at a fine taxonomic resolution. Microbial phylotypes are typically defined using a 97% small subunit (16S) rRNA threshold. However, increasing e...

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Bibliographic Details
Main Authors: Chengfeng Yang, Qinzhi Su, Min Tang, Shiqi Luo, Hao Zheng, Xue Zhang, Xin Zhou
Format: Article
Language:English
Published: American Society for Microbiology 2022-04-01
Series:Microbiology Spectrum
Subjects:
microbiota
SDP
quantification
16S V4 region
metagenomics
Gilliamella
Online Access:https://journals.asm.org/doi/10.1128/spectrum.02105-21

Internet

https://journals.asm.org/doi/10.1128/spectrum.02105-21

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