Single-cell RNA sequencing of murine ankle joints over time reveals distinct transcriptional changes following Borrelia burgdorferi infection
Summary: Lyme disease is caused by the bacterial pathogen Borrelia burgdorferi, which can be readily modeled in laboratory mice. In order to understand the cellular and transcriptional changes that occur during B. burgdorferi infection, we conducted single-cell RNA sequencing (scRNA-seq) of ankle jo...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223022940 |
| _version_ | 1827782317547978752 |
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| author | Jennifer D. Helble Michael J. Walsh Julie E. McCarthy Neal P. Smith Alice J. Tirard Benjamin Y. Arnold Alexandra-Chloe Villani Linden T. Hu |
| author_facet | Jennifer D. Helble Michael J. Walsh Julie E. McCarthy Neal P. Smith Alice J. Tirard Benjamin Y. Arnold Alexandra-Chloe Villani Linden T. Hu |
| author_sort | Jennifer D. Helble |
| collection | DOAJ |
| description | Summary: Lyme disease is caused by the bacterial pathogen Borrelia burgdorferi, which can be readily modeled in laboratory mice. In order to understand the cellular and transcriptional changes that occur during B. burgdorferi infection, we conducted single-cell RNA sequencing (scRNA-seq) of ankle joints of infected C57BL/6 mice over time. We found that macrophages/monocytes, T cells, synoviocytes and fibroblasts all showed significant differences in gene expression of both inflammatory and non-inflammatory genes that peaked early and returned to baseline before the typical resolution of arthritis. Predictions of cellular interactions showed that macrophages appear to communicate extensively between different clusters of macrophages as well as with fibroblasts and synoviocytes. Our data give unique insights into the interactions between B. burgdorferi and the murine immune system over time and allow for a better understanding of mechanisms by which the dysregulation of the immune response may lead to prolonged symptoms in some patients. |
| first_indexed | 2024-03-11T15:31:35Z |
| format | Article |
| id | doaj.art-d6183220150540f2ace8e1063c19b325 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-11T15:31:35Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-d6183220150540f2ace8e1063c19b3252023-10-27T04:24:34ZengElsevieriScience2589-00422023-11-012611108217Single-cell RNA sequencing of murine ankle joints over time reveals distinct transcriptional changes following Borrelia burgdorferi infectionJennifer D. Helble0Michael J. Walsh1Julie E. McCarthy2Neal P. Smith3Alice J. Tirard4Benjamin Y. Arnold5Alexandra-Chloe Villani6Linden T. Hu7Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USADivision of Gastroenterology, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Cancer Immunology and Virology, Dana Farber Cancer Institute, Boston, MA 02215, USADepartment of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USADepartment of Medicine, Massachusetts General Hospital, Boston, MA 02114, USADepartment of Medicine, Massachusetts General Hospital, Boston, MA 02114, USADepartment of Medicine, Massachusetts General Hospital, Boston, MA 02114, USADepartment of Medicine, Massachusetts General Hospital, Boston, MA 02114, USADepartment of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA; Corresponding authorSummary: Lyme disease is caused by the bacterial pathogen Borrelia burgdorferi, which can be readily modeled in laboratory mice. In order to understand the cellular and transcriptional changes that occur during B. burgdorferi infection, we conducted single-cell RNA sequencing (scRNA-seq) of ankle joints of infected C57BL/6 mice over time. We found that macrophages/monocytes, T cells, synoviocytes and fibroblasts all showed significant differences in gene expression of both inflammatory and non-inflammatory genes that peaked early and returned to baseline before the typical resolution of arthritis. Predictions of cellular interactions showed that macrophages appear to communicate extensively between different clusters of macrophages as well as with fibroblasts and synoviocytes. Our data give unique insights into the interactions between B. burgdorferi and the murine immune system over time and allow for a better understanding of mechanisms by which the dysregulation of the immune response may lead to prolonged symptoms in some patients.http://www.sciencedirect.com/science/article/pii/S2589004223022940ImmunologyMicrobiologyCell biologyTranscriptomics |
| spellingShingle | Jennifer D. Helble Michael J. Walsh Julie E. McCarthy Neal P. Smith Alice J. Tirard Benjamin Y. Arnold Alexandra-Chloe Villani Linden T. Hu Single-cell RNA sequencing of murine ankle joints over time reveals distinct transcriptional changes following Borrelia burgdorferi infection iScience Immunology Microbiology Cell biology Transcriptomics |
| title | Single-cell RNA sequencing of murine ankle joints over time reveals distinct transcriptional changes following Borrelia burgdorferi infection |
| title_full | Single-cell RNA sequencing of murine ankle joints over time reveals distinct transcriptional changes following Borrelia burgdorferi infection |
| title_fullStr | Single-cell RNA sequencing of murine ankle joints over time reveals distinct transcriptional changes following Borrelia burgdorferi infection |
| title_full_unstemmed | Single-cell RNA sequencing of murine ankle joints over time reveals distinct transcriptional changes following Borrelia burgdorferi infection |
| title_short | Single-cell RNA sequencing of murine ankle joints over time reveals distinct transcriptional changes following Borrelia burgdorferi infection |
| title_sort | single cell rna sequencing of murine ankle joints over time reveals distinct transcriptional changes following borrelia burgdorferi infection |
| topic | Immunology Microbiology Cell biology Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223022940 |
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