The effects of the heme precursor 5-aminolevulinic acid (ALA) on REV-ERBα activation
The nuclear receptor, REV-ERBα, has a key role in circadian rhythms and requires heme as its ligand. The present study determined whether the heme precursor, 5-aminolevulinic acid (ALA), affects REV-ERBα and its target genes. When exposed to ALA, the human lung diploid cell line, WI-38, exhibited ac...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211546314000333 |
| _version_ | 1818502010761117696 |
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| author | Kohei Yamashita Yuichiro Hagiya Motowo Nakajima Masahiro Ishizuka Tohru Tanaka Shun-ichiro Ogura |
| author_facet | Kohei Yamashita Yuichiro Hagiya Motowo Nakajima Masahiro Ishizuka Tohru Tanaka Shun-ichiro Ogura |
| author_sort | Kohei Yamashita |
| collection | DOAJ |
| description | The nuclear receptor, REV-ERBα, has a key role in circadian rhythms and requires heme as its ligand. The present study determined whether the heme precursor, 5-aminolevulinic acid (ALA), affects REV-ERBα and its target genes. When exposed to ALA, the human lung diploid cell line, WI-38, exhibited activation of REV-ERBα and repression of the transcription of REV-ERBα target genes, including BMAL1, an essential component of the circadian oscillator. Moreover, co-incubation of sodium ferrous citrate (SFC) and ALA also activated REV-ERBα and repressed the transcription of REV-ERBα target genes. These results indicate that ALA regulates human circadian rhythms via REV-ERBα. |
| first_indexed | 2024-12-10T21:04:05Z |
| format | Article |
| id | doaj.art-d61b5c701cc74a5590d673a259399bf1 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-12-10T21:04:05Z |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-d61b5c701cc74a5590d673a259399bf12022-12-22T01:33:41ZengWileyFEBS Open Bio2211-54632014-01-014C34735210.1016/j.fob.2014.03.010The effects of the heme precursor 5-aminolevulinic acid (ALA) on REV-ERBα activationKohei Yamashita0Yuichiro Hagiya1Motowo Nakajima2Masahiro Ishizuka3Tohru Tanaka4Shun-ichiro Ogura5Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B102, Nagatsuta-cho, Midori-ku, Yokohama 226-8501, JapanGraduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B102, Nagatsuta-cho, Midori-ku, Yokohama 226-8501, JapanSBI Pharmaceuticals CO., LTD., Izumi Garden Tower 20F, 1-6-1, Roppongi, Minato-ku, Tokyo 106-6020, JapanSBI Pharmaceuticals CO., LTD., Izumi Garden Tower 20F, 1-6-1, Roppongi, Minato-ku, Tokyo 106-6020, JapanSBI Pharmaceuticals CO., LTD., Izumi Garden Tower 20F, 1-6-1, Roppongi, Minato-ku, Tokyo 106-6020, JapanGraduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B102, Nagatsuta-cho, Midori-ku, Yokohama 226-8501, JapanThe nuclear receptor, REV-ERBα, has a key role in circadian rhythms and requires heme as its ligand. The present study determined whether the heme precursor, 5-aminolevulinic acid (ALA), affects REV-ERBα and its target genes. When exposed to ALA, the human lung diploid cell line, WI-38, exhibited activation of REV-ERBα and repression of the transcription of REV-ERBα target genes, including BMAL1, an essential component of the circadian oscillator. Moreover, co-incubation of sodium ferrous citrate (SFC) and ALA also activated REV-ERBα and repressed the transcription of REV-ERBα target genes. These results indicate that ALA regulates human circadian rhythms via REV-ERBα.http://www.sciencedirect.com/science/article/pii/S22115463140003335-Aminolevulinic acidCircadian rhythmsREV-ERBαPorphyrin metabolism |
| spellingShingle | Kohei Yamashita Yuichiro Hagiya Motowo Nakajima Masahiro Ishizuka Tohru Tanaka Shun-ichiro Ogura The effects of the heme precursor 5-aminolevulinic acid (ALA) on REV-ERBα activation FEBS Open Bio 5-Aminolevulinic acid Circadian rhythms REV-ERBα Porphyrin metabolism |
| title | The effects of the heme precursor 5-aminolevulinic acid (ALA) on REV-ERBα activation |
| title_full | The effects of the heme precursor 5-aminolevulinic acid (ALA) on REV-ERBα activation |
| title_fullStr | The effects of the heme precursor 5-aminolevulinic acid (ALA) on REV-ERBα activation |
| title_full_unstemmed | The effects of the heme precursor 5-aminolevulinic acid (ALA) on REV-ERBα activation |
| title_short | The effects of the heme precursor 5-aminolevulinic acid (ALA) on REV-ERBα activation |
| title_sort | effects of the heme precursor 5 aminolevulinic acid ala on rev erbα activation |
| topic | 5-Aminolevulinic acid Circadian rhythms REV-ERBα Porphyrin metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2211546314000333 |
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