Perfusion culture maintained with an air-liquid interface to stimulate epithelial cell organization in renal organoids in vitro

Perfusion culture maintained with an air-liquid interface to stimulate epithelial cell organization in renal organoids in vitro

Abstract Background Organoids derived from induced pluripotent stem (iPS) or embryonic stem (ES) cells have been evaluated as in vitro models of development and disease. However, maintaining these cells under long-term static culture conditions is difficult because of nutrition shortages and waste a...

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Bibliographic Details
Main Authors: Sachiko Sekiya, Tetsutaro Kikuchi, Tatsuya Shimizu
Format: Article
Language:English
Published: BMC 2019-07-01
Series:BMC Biomedical Engineering
Subjects:
Renal organoid
Perfusion culture
Air-liquid interface
Online Access:http://link.springer.com/article/10.1186/s42490-019-0017-9

Internet

http://link.springer.com/article/10.1186/s42490-019-0017-9

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