Targeted, homology-driven gene insertion in stem cells by ZFN-loaded ‘all-in-one’ lentiviral vectors

Targeted, homology-driven gene insertion in stem cells by ZFN-loaded ‘all-in-one’ lentiviral vectors

Biased integration remains a key challenge for gene therapy based on lentiviral vector technologies. Engineering of next-generation lentiviral vectors targeting safe genomic harbors for insertion is therefore of high relevance. In a previous paper (Cai et al., 2014a), we showed the use of integrase-...

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Bibliographic Details
Main Authors: Yujia Cai, Anders Laustsen, Yan Zhou, Chenglong Sun, Mads Valdemar Anderson, Shengting Li, Niels Uldbjerg, Yonglun Luo, Martin R Jakobsen, Jacob Giehm Mikkelsen
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2016-06-01
Series:eLife
Subjects:
protein transduction
zinc-finger nucleases
iPSC
CD34+ cells
lentiviral vector
Online Access:https://elifesciences.org/articles/12213

Internet

https://elifesciences.org/articles/12213

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