A CRISPR-guided mutagenic DNA polymerase strategy for the detection of antibiotic-resistant mutations in M. tuberculosis

A CRISPR-guided mutagenic DNA polymerase strategy for the detection of antibiotic-resistant mutations in M. tuberculosis

A sharp increase in multidrug-resistant tuberculosis (MDR-TB) threatens human health. Spontaneous mutation in essential gene confers an ability of Mycobacterium tuberculosis resistance to anti-TB drugs. However, conventional laboratory strategies for identification and prediction of the mutations in...

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Bibliographic Details
Main Authors: Siyuan Feng, Lujie Liang, Cong Shen, Daixi Lin, Jiachen Li, Lingxuan Lyu, Wanfei Liang, Lan-lan Zhong, Gregory M. Cook, Yohei Doi, Cha Chen, Guo-bao Tian
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
CRISPR
Mycobacterium tuberculosis
drug resistance
fitness
mutation
high-throughput sequencing
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253122001731

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http://www.sciencedirect.com/science/article/pii/S2162253122001731

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