Development of an Event-Specific Droplet Digital PCR Assay for Quantification and Evaluation of the Transgene DNAs in Trace Samples of GM <i>PRNP</i>-Knockout Goat

Development of an Event-Specific Droplet Digital PCR Assay for Quantification and Evaluation of the Transgene DNAs in Trace Samples of GM <i>PRNP</i>-Knockout Goat

The prion protein (<i>PRNP</i>) gene encoding prion protein is considered a prerequisite for the occurrence of scrapie disease, and knockout of the <i>PRNP</i> gene in transgenic goat is one effective approach to avoid scrapie. This study aims to establish an event-specific d...

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Bibliographic Details
Main Authors: Wenting Xu, Ping Shen, Rong Li, Biao Liu, Litao Yang
Format: Article
Language:English
Published: MDPI AG 2022-03-01
Series:Foods
Subjects:
ddPCR
genetically modified <i>PRNP</i>-knockout goat
event-specific
trace samples
Online Access:https://www.mdpi.com/2304-8158/11/6/868

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https://www.mdpi.com/2304-8158/11/6/868

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