Cloning of the gene encoding Endo-β-1,3-1,4 Glucanase from Bacillus subtilis subsp. spizizenii W23 on Plasmid pMMB67EH into Escherichia coli DH5α and Escherichia coli Origami

One of the genes encoding cellulolytic enzymes, named BSUW23_10175, from Bacillus subtilis subsp. spizizenii W23 was cloned in the plasmid pMMB67EH into Escherichia coli DH5α and Escherichia coli Origami. Target gene was amplified using polymerase chain reaction (PCR) with specific primers (For- BSU...

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Bibliographic Details
Main Author: Lisa Gunawan
Format: Article
Language:Indonesian
Published: Universitas Gadjah Mada 2015-11-01
Series:Jurnal Sain Veteriner
Subjects:
Online Access:https://jurnal.ugm.ac.id/jsv/article/view/8114