Single-cell isolation from full-thickness human intestinal tissue resections for single-cell RNA sequencing

Single-cell isolation from full-thickness human intestinal tissue resections for single-cell RNA sequencing

Summary: Single-cell isolation techniques allow the investigation of physical and functional relationships between individual cells within a complex cell population. Here, we present a protocol for single-cell isolation from full-thickness intestinal tissue resections. We describe steps for pre-proc...

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Bibliographic Details
Main Authors: Gail A. West, Shuai Zhao, Quang Tam Nguyen, Stephen M. Christensen, Ilyssa O. Gordon, Stefan D. Holubar, Kellie M. Kravarik, Claudio Fiocchi, Pranab K. Mukherjee, Florian Rieder
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Cell isolation
Single Cell
Cell separation/fractionation
RNA-seq
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723006536

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http://www.sciencedirect.com/science/article/pii/S2666166723006536

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