Protocol for generating airway organoids from 2D air liquid interface-differentiated nasal epithelia for use in a functional CFTR assay

Protocol for generating airway organoids from 2D air liquid interface-differentiated nasal epithelia for use in a functional CFTR assay

Summary: We present a protocol to generate organoids from air-liquid-interface (ALI)-differentiated nasal epithelia. We detail their application as cystic fibrosis (CF) disease model in the cystic fibrosis transmembrane conductance regulator (CFTR)-dependent forskolin-induced swelling (FIS) assay. W...

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Bibliographic Details
Main Authors: Lisa W. Rodenburg, Isabelle S. van der Windt, Henriette H.M. Dreyer, Shannon M.A. Smits, Loes A. den Hertog - Oosterhoff, Ellen M. Aarts, Jeffrey M. Beekman, Gimano D. Amatngalim
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Cell culture
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723003040

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http://www.sciencedirect.com/science/article/pii/S2666166723003040

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