Extraction, Bacteriostatic Effect and Synergistic Mechanism of Bacteriostatic Effect of Taxifolin in Larch in Combination with Leucocyanidin
Using larch as raw material, taxifolin was extracted by ultrasonic-assisted ethanol hot leaching. Escherichia coli and Staphylococcus aureus were used as typical test bacteria respectively, and the bacterial inhibitory effects of taxifolin was analyzed by observing the morphological structure of bac...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2024-01-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022110270 |
| _version_ | 1797376664924061696 |
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| author | Licheng JING Yue YANG Kuo YANG Meixuan LI Min ZHAO Daizong CUI |
| author_facet | Licheng JING Yue YANG Kuo YANG Meixuan LI Min ZHAO Daizong CUI |
| author_sort | Licheng JING |
| collection | DOAJ |
| description | Using larch as raw material, taxifolin was extracted by ultrasonic-assisted ethanol hot leaching. Escherichia coli and Staphylococcus aureus were used as typical test bacteria respectively, and the bacterial inhibitory effects of taxifolin was analyzed by observing the morphological structure of bacteria and measuring the changes in bacterial growth, cell membrane leakage and antioxidant enzyme system activities. The final product was obtained with 90% purity and 0.35% extraction rate of taxifolin. The minimum inhibitory concentration of taxifolin was 1.2 mg/mL for both strains, and the inhibition rate was 81.12% and 83.95%, respectively. After the electron microscopy analysis, the surface of the bacteria cells was disrupted and the contents inside the cells were leaked. In E. coli, the OD260 test values of 1/2 MIC, 1 MIC and 2 MIC were 1.18, 1.52 and 1.88 times higher than those of the control group after 24 h incubation. The relative activities of extracellular β-galactosidase were 79.15% and 70.1%, respectively, and the growth rate calculated by the the Bliss independent model was 0.0142. S. aureus showed a synergistic inhibition effect. Under the conditions of drug combination, the bacterial cell membrane disruption extent was significantly enhanced by the low concentration and the inhibition effect was more significant. The β-galactosidase activity in the culture supernatant was 87.01% higher than that of 2 MIC group alone for S. aureus by 16.91%, and the 24 h CAT, SOD and POD enzyme activities were higher than that of 1 MIC group alone for S. aureus, which were 1.28, 1.25 and 1.11 times higher, respectively. The study could lay the theoretical foundation for the development and application of taxifolin as a food preservation and preservative. |
| first_indexed | 2024-03-08T19:41:59Z |
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| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-08T19:41:59Z |
| publishDate | 2024-01-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-d6de83516ddf4cb09f7dd094e2b3479d2023-12-25T05:30:37ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062024-01-0145112813610.13386/j.issn1002-0306.20221102702022110270-1Extraction, Bacteriostatic Effect and Synergistic Mechanism of Bacteriostatic Effect of Taxifolin in Larch in Combination with LeucocyanidinLicheng JING0Yue YANG1Kuo YANG2Meixuan LI3Min ZHAO4Daizong CUI5College of Life Sciences, Northeast Forestry University, Harbin 150036, ChinaCollege of Life Sciences, Northeast Forestry University, Harbin 150036, ChinaCollege of Life Sciences, Northeast Forestry University, Harbin 150036, ChinaCollege of Life Sciences, Northeast Forestry University, Harbin 150036, ChinaCollege of Life Sciences, Northeast Forestry University, Harbin 150036, ChinaCollege of Life Sciences, Northeast Forestry University, Harbin 150036, ChinaUsing larch as raw material, taxifolin was extracted by ultrasonic-assisted ethanol hot leaching. Escherichia coli and Staphylococcus aureus were used as typical test bacteria respectively, and the bacterial inhibitory effects of taxifolin was analyzed by observing the morphological structure of bacteria and measuring the changes in bacterial growth, cell membrane leakage and antioxidant enzyme system activities. The final product was obtained with 90% purity and 0.35% extraction rate of taxifolin. The minimum inhibitory concentration of taxifolin was 1.2 mg/mL for both strains, and the inhibition rate was 81.12% and 83.95%, respectively. After the electron microscopy analysis, the surface of the bacteria cells was disrupted and the contents inside the cells were leaked. In E. coli, the OD260 test values of 1/2 MIC, 1 MIC and 2 MIC were 1.18, 1.52 and 1.88 times higher than those of the control group after 24 h incubation. The relative activities of extracellular β-galactosidase were 79.15% and 70.1%, respectively, and the growth rate calculated by the the Bliss independent model was 0.0142. S. aureus showed a synergistic inhibition effect. Under the conditions of drug combination, the bacterial cell membrane disruption extent was significantly enhanced by the low concentration and the inhibition effect was more significant. The β-galactosidase activity in the culture supernatant was 87.01% higher than that of 2 MIC group alone for S. aureus by 16.91%, and the 24 h CAT, SOD and POD enzyme activities were higher than that of 1 MIC group alone for S. aureus, which were 1.28, 1.25 and 1.11 times higher, respectively. The study could lay the theoretical foundation for the development and application of taxifolin as a food preservation and preservative.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022110270taxifolinantibacterial activitydrug combinationmechanism of inhibition |
| spellingShingle | Licheng JING Yue YANG Kuo YANG Meixuan LI Min ZHAO Daizong CUI Extraction, Bacteriostatic Effect and Synergistic Mechanism of Bacteriostatic Effect of Taxifolin in Larch in Combination with Leucocyanidin Shipin gongye ke-ji taxifolin antibacterial activity drug combination mechanism of inhibition |
| title | Extraction, Bacteriostatic Effect and Synergistic Mechanism of Bacteriostatic Effect of Taxifolin in Larch in Combination with Leucocyanidin |
| title_full | Extraction, Bacteriostatic Effect and Synergistic Mechanism of Bacteriostatic Effect of Taxifolin in Larch in Combination with Leucocyanidin |
| title_fullStr | Extraction, Bacteriostatic Effect and Synergistic Mechanism of Bacteriostatic Effect of Taxifolin in Larch in Combination with Leucocyanidin |
| title_full_unstemmed | Extraction, Bacteriostatic Effect and Synergistic Mechanism of Bacteriostatic Effect of Taxifolin in Larch in Combination with Leucocyanidin |
| title_short | Extraction, Bacteriostatic Effect and Synergistic Mechanism of Bacteriostatic Effect of Taxifolin in Larch in Combination with Leucocyanidin |
| title_sort | extraction bacteriostatic effect and synergistic mechanism of bacteriostatic effect of taxifolin in larch in combination with leucocyanidin |
| topic | taxifolin antibacterial activity drug combination mechanism of inhibition |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022110270 |
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